Oxyntomodulin peptide analogue
The present invention provides Oxyntomodulin peptide analogues useful in the treatment of diabetes and/or obesity.
1. An Oxyntomodulin peptide analogue comprising the amino acid sequence:
(SEQ ID NO: 5)
His-(D-Ser)-Gln-Gly-Thr-Phe-Thr-Ser-Asp-(1-Nal)-
Ser-Lys-Tyr-Leu-Asp-Glu-Lys-Ala-Ala-Gln-Glu-Phe-
Val-Gln-Trp-Leu-Leu-Asn-(Aib)-Ala-Arg-Asn-Arg-
Asn-Asn-Ile-Ala-Xaa 38 -Xaa 39
wherein Xaa 38 is Cys, Cys-PEG, or is absent; Xaa 39 is Cys, Cys-PEG, or is absent;
and wherein the C-terminal amino acid is optionally amidated.
2. The Oxyntomodulin peptide analogue according to claim 1 , comprising the amino acid sequence:
(SEQ ID NO: 2)
His-(D-Ser)-Gln-Gly-Thr-Phe-Thr-Ser-Asp-(1-Nal)-
Ser-Lys-Tyr-Leu-Asp-Glu-Lys-Ala-Ala-Gln-Glu-Phe-
Val-Gln-Trp-Leu-Leu-Asn-(Aib)-Ala-Arg-Asn-Arg-
Asn-Asn-Ile-Ala-Cys-Cys
wherein the Cys residue at position 38 is optionally PEGylated; and wherein the Cys residue at position 39 is optionally PEGylated.
3. The Oxyntomodulin peptide analogue according to claim 2 , wherein the analogue is PEGylated on the thiol of the Cys residue at either position 38 or position 39 with an approximately 40 kDa PEG molecule.
4. The Oxyntomodulin peptide analogue according to claim 2 , wherein the analogue is PEGylated on the thiol of both Cys residues at positions 38 and 39 with an approximately 20 kDa PEG molecule in each case and comprises the amino acid sequence:
(SEQ ID NO: 3)
His-(D-Ser)-Gln-Gly-Thr-Phe-Thr-Ser-Asp-(1-Nal)-
Ser-Lys-Tyr-Leu-Asp-Glu-Lys-Ala-Ala-Gln-Glu-Phe-
Val-Gln-Trp-Leu-Leu-Asn-(Aib)-Ala-Arg-Asn-Arg-
Asn-Asn-Ile-Ala-Cys(PEG20k)-Cys(PEG20k)
5. The Oxyntomodulin peptide analogue according to claim 4 , wherein each PEG molecule is linear.
6. The Oxyntomodulin peptide analogue according to claim 5 , wherein each PEG molecule is attached to the Cys residue by an maleimide linker moiety.
7. The Oxyntomodulin peptide analogue according to claim 6 , wherein the carboxyl group of the Cys residue at position 39 is amidated.
8. A method of treating obesity in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 7 .
9. The Oxyntomodulin peptide analogue according to claim 1 , wherein the Cys residue at position 39 is absent, and the Cys residue at position 38 is PEGylated with an approximately 40 kDa PEG molecule.
10. A pharmaceutical composition comprising the Oxyntomodulin peptide analogue of claim 1 , and a pharmaceutically acceptable carrier, diluent, or excipient.
11. A method of treating non-insulin-dependent diabetes in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 1 .
12. A method of treating obesity in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 1 .
13. A pharmaceutical composition comprising the Oxyntomodulin peptide analogue of claim 7 , and a pharmaceutically acceptable carrier, diluent, or excipient.
14. A method of treating non-insulin-dependent diabetes in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 7 .
15. An Oxyntomodulin peptide analogue according to Formula 3
16. A pharmaceutical composition comprising the Oxyntomodulin peptide analogue of claim 15 , and a pharmaceutically acceptable carrier, diluent, or excipient.
17. A method of treating non-insulin-dependent diabetes in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 15 .
18. A method of treating obesity in a subject in need thereof, comprising administering to the subject an effective amount of an Oxyntomodulin peptide analogue according to claim 15 .