Antimicrobial wash and carrier solutions and uses thereof
Novel wash and carrier solutions ideally suited for topical administration (e.g., as wash solutions and as carrier solutions). The solutions described herein are non-toxic, non-irritating, isotonic, possess non-specific broad-spectrum antimicrobial properties, and have an alkaline pH. Also disclosed are sterile preparations of the solutions and methods of using the same.
1. A method of washing a wound comprising washing the wound with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
2. The method of claim 1 , wherein the buffer system is a Tris buffer system comprising tromethamine base at a concentration of about 0.3 to about 0.55% (w/w) and tromethamine hydrochloride at a concentration of about 0.3 to about 0.45% (w/w).
3. The method of claim 1 , wherein the surfactant is selected from the group consisting of nonoxynol 12, PPG-12/PEG-50 lanolin, phospholipid CDM, phospholipid EFA, phospholipid PTA, PEG-100 stearate, and any other nonoxynol, octoxynol surfactants with different PEG chain lengths.
4. The method of claim 1 , wherein the metal ion chelating agent is selected from the group consisting of disodium EDTA, tetrasodium EDTA, lauroyl EDTA, dilauroyl EDTA and edetate calcium disodium.
5. A method of treating a topical microbial infection of a tissue comprising washing the tissue with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
6. A method of preparing a tissue for surgery comprising washing the tissue prior to the surgery with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
7. A method of topically delivering a pharmaceutical agent to a tissue comprising:
(a) mixing the pharmaceutical with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(i) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(ii) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(iii) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(iv) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating; and
(b) applying the resulting mixture to the tissue.
8. A method of washing a wound comprising washing the wound with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8.2 to about 8.8;
(b) a surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts at a concentration of about 0.05 wt. % to about 0.15 wt. %; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol at a concentration of about 0.5% (w/w) to about 2% (w/w),
wherein the solution has a pH in the range of about 8.2 to about 8.8 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.