Synthetic mycotoxin adsorbents and methods of making and utilizing the same

1. A method of sequestering a mycotoxin from a material, comprising:

a) providing:

i) a material comprising mycotoxins; and

ii) a molecularly imprinted polymer generated via polymerization of one or more monomers and one or more crosslinkers in the presence of a mycotoxin template selected from the group consisting of: N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine; 5-chloro-6,7-dimethoxy-1-methylisatine; ethyl 3-hydroxy-6,7-dimethoxy-2-indolone-3-carboxylate; 6,7-dimethoxyisatine; and 6,7-dimethoxy-1-methylisatine; and

b) contacting said molecularly imprinted polymer with said material comprising mycotoxins under conditions that permit said molecularly imprinted polymer to bind said mycotoxin.

2. The method of claim 1 , wherein ochratoxin A is sequestered from said material comprising mycotoxins.

3. The method of claim 1 , wherein sporidesmin is sequestered from said material comprising mycotoxins.

4. The method of claim 1 , wherein said material comprising mycotoxins is selected from the group consisting of a beverage, a foodstuff, an animal feed, a pharmaceutical composition, a nutraceutical composition, a cosmetic composition, and a substance necessary to sustain life.

5. The method of claim 4 , wherein said substance necessary to sustain life is selected from the group consisting of a medium for use in aquaculture and a gaseous sample comprising oxygen.

6. The method of claim 1 , wherein a molecularly imprinted polymer bound to a mycotoxin is not separated from said material comprising mycotoxins.

7. The method of claim 1 , further comprising c) separating bound mycotoxin from said molecularly imprinted polymers.

8. The method of claim 7 , wherein said separating comprises extracting, concentrating or isolating said mycotoxin from said material and from said molecularly imprinted polymers.

9. The method of claim 8 , wherein said separating occurs in a chromatographic or separating column or cartridge.

10. The method of claim 8 , wherein after separating, mycotoxin bound to said molecularly imprinted polymers are removed from said molecularly imprinted polymers by washing.

11. The method of claim 10 , wherein the mycotoxins are qualitatively or quantitatively analyzed after removal from said molecularly imprinted polymers.

12. The method of claim 11 , wherein the quantitative and qualitative analysis is utilized for traceability.

13. The method of claim 10 , wherein said molecularly imprinted polymer from which mycotoxins has been removed is reused to sequester a mycotoxin from a material comprising mycotoxins.

14. The method of claim 1 , wherein said molecularly imprinted polymer adsorbs 1 to 10 times more water than its weight.

15. The method of claim 14 , wherein said molecularly imprinted polymer adsorbs 1 to 5 times more water than its weight.

16. The method of claim 14 , wherein said molecularly imprinted polymer adsorbs 1 to 2 times more water than its weight.

17. The method of claim 1 , wherein two or more different molecularly imprinted polymers are contacted with said material comprising mycotoxins in order to sequester two or more different types of mycotoxins from said material.

18. A method of making a molecularly imprinted polymer comprising:

a) providing:

i. a mycotoxin template selected from the group consisting of: N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine; 5-chloro-6,7-dimethoxy-1 methylisatine; ethyl 3-hydroxy-6,7-dimethoxy-2-indolone-3-carboxylate; 6,7-dimethoxyisatine; and 6,7-dimethoxy-1-methylisatine; and

ii. one or more monomers and one or more crosslinkers; and

b) contacting said mycotoxin template with said one or more monomers and said one or more crosslinkers under conditions that permit polymerization of said one or more monomers and said one or more crosslinkers in the presence of said mycotoxin template.

19. The method of claim 18 , wherein said mycotoxin template is N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine.

20. The method of claim 18 , wherein said mycotoxin template is 5-chloro-6,7-dimethoxy-1-methylisatine.

21. The method of claim 18 , wherein said one or more monomers are selected from the group consisting of 2-vinylpyridine, 2-hydroxyethylmethacrylate and methacrylic acid.

22. The method of claim 18 , wherein said one or more crosslinkers comprise ethylene glycol dimethacrylate.

23. The method of claim 18 , wherein said polymerization is initiated at low temperature by UV light.

24. The method of claim 18 , wherein said polymerization is initiated by forming free radicals in an organic solvent at a temperature between 55 and 110 degrees Celsius.

25. The method of claim 24 , wherein said free radicals are formed by thermally initiated decomposition of azoisobutyronitrile (AIBN).

26. The method of claim 24 , wherein said organic solvent is selected from the group consisting of toluene, cyclohexane, acetonitrile, a polyvinyl alcohol (PVA)/water solution, and a mixture of two or more of toluene, cyclohexane, acetonitrile, and a PVA/water solution.

27. The method of claim 24 , wherein said temperature is between 55 and 75 degrees Celsius.

28. The method of claim 7 wherein said mycotoxin template is removed from said molecularly imprinted polymer after polymerization of said one or more monomers and said one or more crosslinkers.

29. The method of claim 28 , wherein one or more washes with a solution selected from the group consisting of an organic solvent, a buffer, water and a combination thereof is utilized to remove said mycotoxin template from said molecularly imprinted polymer.

30. The method of claim 29 , wherein said organic solvent is selected from the group consisting of ethyl alcohol, methyl alcohol, acetonitrile, toluene, and a mixture thereof.

31. The method of claim 29 wherein said buffer is a buffer prepared by reacting sodium hydroxide, citric acid, succinic acid and acetic acid.

32. The method of claim 29 , wherein said molecularly imprinted polymer is dried after said one or more washes.