Nucleic acids encoding TNFγ
Nucleic acids encoding mammalian, e.g., primate or rodent, genes, purified proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are provided.
1. An isolated nucleic acid encoding a polypeptide comprising the sequence of SEQ ID NO: 13.
2. An expression vector comprising the nucleic acid of claim 1 .
3. A host cell comprising the expression vector of claim 2 .
4. The host cell of claim 3 , where in the host cell is selected from the group consisting of a bacterial cell, a mammalian cell, and a yeast cell.
5. The host cell of claim 4 , wherein the mammalian cell is a CHO cell.
6. A method of expressing the polypeptide of SEQ ID NO: 13, comprising:
a) culturing the host cell of claim 3 in a medium suitable for expression of the polypeptide; and
b) isolating the polypeptide.
7. The nucleic acid of claim 1 , wherein the nucleic acid has a sequence of SEQ ID NO: 12.