Plasma separation
Devices and methods for separating plasma from biological fluids such as blood and blood products are disclosed.
1. A plasma separation device comprising:
(a) a filter having an upstream surface and a downstream surface, the filter comprising a microporous membrane; and,
(b) a housing, having an inlet, a downstream chamber, and an outlet, the housing comprising a base comprising the downstream chamber and the outlet and two or more air channels, wherein the base further comprises at least one plasma channel in fluid communication with the outlet; defining a fluid flow path between the inlet, the downstream chamber, and the outlet, with the filter disposed in the housing across the fluid flow path, wherein the outlet and the air channels are spaced apart and are arranged such that, when a negative pressure is created in the downstream chamber through the outlet, air passes into the downstream chamber via the air channels and sweeps plasma from the downstream surface of the filter and through the outlet.
2. The device of claim 1 , wherein the outlet and at least one air channel are respectively disposed near opposite ends of the base.
3. The device of claim 1 , wherein the membrane is an asymmetrical membrane having larger openings at the upstream surface than at the downstream surface.
4. The device of claim 1 , wherein filter includes a plurality of porous media.
5. The device of claim 4 , wherein the filter includes the membrane and a fibrous medium.
6. The device of claim 5 , wherein the fibrous medium includes glass fibers.
7. The device of claim 5 , wherein the fibrous medium includes melt-blown fibers.
8. A method of separating plasma from biological fluid, the method comprising:
obtaining a plasma separation device comprising a filter having an upstream surface and a downstream surface, the filter comprising a microporous membrane; and, a housing, having an inlet, a downstream chamber, and an outlet, and at least one air channel; defining a fluid flow path between the inlet, the downstream chamber, and the outlet, with the filter disposed in the housing across the fluid flow path, wherein the outlet and the air channel are spaced apart and are arranged such that, when a negative pressure is created in the downstream chamber through the outlet, air passes into the downstream chamber via the air channel and sweeps plasma from the downstream surface of the filter and through the outlet; and,
applying biological fluid to the upstream surface of the filter;
passing plasma from the upstream surface of the filter to the downstream surface of the filter;
creating a negative pressure in the downstream chamber through the outlet;
passing air through the air channel into the downstream chamber and sweeping plasma from the downstream surface of the filter; and,
passing swept plasma through the outlet.
9. The method of claim 8 , wherein the plasma separation device comprises a housing comprising a base comprising the downstream chamber and the outlet and two or more air channels, the base further comprising at least one plasma channel in fluid communication with the outlet, wherein the outlet and the air channels are spaced apart; the negative pressure including passing air through the air channels into the downstream chamber and sweeping plasma from the downstream surface of the filter; and, passing swept plasma through the outlet.