Biomolecular coating for implants
Methods and compositions are provided for improving tissue growth and device integration in vivo. Substrates and devices coated with an α 2 β 1 or α 5 β 1 integrin-specific ligand are provided. The substrates and devices coated with an α 2 β 1 or α 5 β 1 integrin-specific ligand are shown to have greater tissue formation on the surface relative to controls, in particular greater bone formation.
1. A gel comprising a peptide mimetic integrin ligand in an amount effective for promoting α 2 β 1 -mediated or α 5 β 1 -mediated osseointegration in vivo between bone and a substrate in a subject in need thereof, wherein the peptide mimetic ligand is formulated into a gel wherein the peptide mimetic ligand is selected from the group consisting of the peptide comprising the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 4, or the peptide consisting of the amino acid sequence of SEQ ID NO: 2, or a combination thereof.
2. The gel of claim 1 , wherein the peptide mimetic integrin ligand is selected from the group consisting of a peptide comprising the amino acid sequence of SEQ ID NO: 1 or the peptide consisting of the amino acid sequence of SEQ ID NO :2, or a combination thereof.
3. The gel of claim 1 , wherein the gel promotes α 2 β 1 -mediated osseointegration between the bone and the substrate.
4. The gel of claim 1 , wherein the gel promotes α 5 β 1 -mediated osseointegration between the bone and the substrate.
5. The gel of claim 1 , wherein the substrate is selected from the group consisting of bone, polymer and metal.
6. The gel of claim 1 , wherein the substrate is a substrate of a medical device.
7. The gel of claim 6 , wherein the medical device is selected from the group consisting of an implant, rod, pin, screw, brace, plate, prosthesis, and a tissue engineering scaffold.
8. The gel of claim 5 , wherein the polymer comprises polycaprolactone.
9. The gel of claim 1 , wherein the peptide mimetic integrin ligand consists of GFOGER (SEQ ID NO:1).
10. The gel of claim 1 , wherein the peptide mimetic integrin ligand consists of FNIII 7-10 (SEQ ID NO:2).
11. A method for treating a bone fracture in a subject in need thereof comprising administering the gel of claim 1 to the bone fracture in the subject, wherein the gel promotes repair of the bone fracture.
12. A method for increasing fusion of bone surfaces in a subject in need thereof comprising administering the gel of claim 1 to the bone surfaces to be fused in the subject, wherein the gel promotes the fusion of the bone surfaces in the subject.
13. A method for treating a bone fracture in a subject in need thereof comprising administering to the subject a medical device in combination with the gel of claim 1 , wherein the combination of the medical device and the gel treat the bone defect.