Microfluidic extraction and reaction device
A microfluidic arrangement for extracting and optionally processing an extract from a sample and for transferring the extract in flowable form to a microfluidic chip using an extractor with a compressible extraction chamber and at least one opening thereof, a reactor that has a reaction chamber, an inlet opening that communicates with the at least one opening of the extractor, wherein the two openings define a flow path between the chambers, an outlet opening for fluidically connecting to the microfluidic chip and a ventilation opening of the reaction chamber, and having a filter arrangement installed in the flow path between the extractor and the reactor. A lab-on-a-chip system with such a microfluidic arrangement and a microfluidic chip that is rigidly connected to the reactor.
1. A microfluidic arrangement for extracting and optionally processing an extract from a sample and for transferring the extract in flowable form to a microfluidic chip, comprising:
an extractor comprising a compressible extraction chamber and at least one opening, wherein the extractor also comprises one or more of a stirring element, an acoustic mixer, a vibration mixer and a convection mixer that utilizes heating or cooling,
a reactor that comprises a reaction chamber, an inlet opening that communicates with the at least one opening of the extractor, wherein the two openings define a flow path between the chambers, an outlet opening for fluidically connecting to the microfluidic chip and a ventilation opening of the reaction chamber, and
a filter arrangement installed in the flow path between the extractor and the reactor.
2. The microfluidic arrangement according to claim 1 , wherein the filter arrangement has at least one filter element and a filter holder connected to the reactor and pressing the filter element against the inlet opening of the reactor.
3. The microfluidic arrangement according to claim 1 , wherein the extractor has a cylinder-piston arrangement enclosing the compressible extraction chamber.
4. The microfluidic arrangement according to claim 3 , wherein the cylinder of the extractor can move relative to the filter arrangement and the reactor and the filter arrangement and/or the reactor are at least partly coordinated with the piston or form the latter.
5. The microfluidic arrangement according to claim 4 , wherein the filter arrangement has at least one filter element and a filter holder connected to the reactor and pressing the filter element against the inlet opening of the reactor, and wherein a sealing element is arranged between one outer wall of the filter holder and one inner wall of the cylinder.
6. The microfluidic arrangement according to claim 1 , wherein the extractor has the stirring element enclosed in the extraction chamber.
7. The microfluidic arrangement according to claim 1 , wherein the reactor is fashioned in the shape of a hollow cylinder.
8. The microfluidic arrangement according to claim 1 , wherein the reactor has a flange for fastening to a microfluidic chip.
9. The microfluidic arrangement according to claim 2 , wherein the extractor has a cylinder-piston arrangement enclosing the compressible extraction chamber, and wherein the cylinder of the extractor can move relative to the filter arrangement and the reactor and the filter arrangement and/or the reactor are at least partly coordinated with the piston or form the latter.
10. The microfluidic arrangement according to claim 9 , wherein a sealing element is arranged between one outer wall of the filter holder and one inner wall of the cylinder; and wherein the extractor has a stirring element enclosed in the extraction chamber.
11. The microfluidic arrangement according to claim 10 , wherein the reactor is fashioned in the shape of a hollow cylinder, and wherein the reactor has a flange for fastening to a microfluidic chip.
12. A lab-on-a-chip system comprising: a microfluidic arrangement comprising an extractor comprising a compressible extraction chamber and at least one opening thereof, a reactor that comprises a reaction chamber, an inlet opening that communicates with the at least one opening of the extractor, wherein the two openings define a flow path between the chambers, an outlet opening for fluidically connecting to the microfluidic chip and a ventilation opening of the reaction chamber, and a filter arrangement installed in the flow path between the extractor and the reactor, and
a microfluidic chip that is firmly connected to the reactor.
13. The lab-on-a-chip system according to claim 12 , wherein at least one channel in the microfluidic chip communicates with the outlet opening of the reactor.
14. The lab-on-a-chip system according to claim 13 , wherein the microfluidic chip has a valve arrangement for an optional connecting or separating of the channel communicating with the outlet opening of the reactor to or from at least one inlet and/or outlet line.
15. The lab-on-a-chip system according to claim 14 , wherein the ventilation opening is provided by a capillary tube in the reaction chamber, and wherein a heating device encloses the reactor at least partially.
16. The lab-on-a-chip system according to claim 15 , wherein the reactor is an integral component of the microfluidic chip, and wherein the reactor is molded as a single piece onto the microfluidic chip.
17. The lab-on-a-chip system according to claim 12 , wherein the ventilation opening is provided by a capillary tube in the reaction chamber.
18. The lab-on-a-chip system according to claim 12 , wherein a heating device encloses the reactor at least partially.
19. The lab-on-a-chip system according to claim 12 , wherein the reactor is an integral component of the microfluidic chip.
20. The lab-on-a-chip system according to claim 19 , wherein the reactor is molded as a single piece onto the microfluidic chip.