Polypeptides having colanic acid-degrading activity
The present disclosure generally relates to polypeptides having colanic acid-degrading activity and methods of using the same. Polynucleotides encoding such polypeptides are also described. The polypeptides may be used, for example, in processes for degrading colanic acid, processes for the removal of endotoxins from biological samples, and processes for purifying plasmid DNA.
1. A purified polypeptide having colanic acid-degrading activity comprising an amino acid sequence having at least 98% homology to SEQ ID NO: 1 or fragment thereof having colanic acid-degrading activity.
2. The polypeptide of claim 1 , wherein the amino acid sequence has at least 99% homology to SEQ ID NO: 1.
3. The polypeptide of claim 1 , further comprising a purification tag.
4. The polypeptide of claim 1 , wherein the amino acid sequence comprises SEQ ID NO: 2.
5. The polypeptide of claim 1 , wherein the amino acid sequence comprises SEQ ID NO: 3.
6. The polypeptide of claim 1 , wherein the amino acid sequence comprises SEQ ID NO: 4.
7. The polypeptide of claim 1 , wherein the amino acid sequence comprises SEQ ID NO: 5.
8. A purified polypeptide having colanic acid-degrading activity comprising an amino acid sequence having at least 98% homology to SEQ ID NO: 2 or a fragment thereof having colanic acid-degrading activity.
9. The polypeptide of claim 8 , further comprising a purification tag.