Method for separating and detecting an analyte
A method for separating and analyzing an analyte is provided which comprises, in a first position, transferring a liquid sample to a multiwell plate with a pipette tip, replacing the tips in the tip rack in the same position, and re-using the pipette tips for aspirating and dispensing liquid.
1. A method for isolating an analyte that may be present in a fluid sample, the method comprising the automated steps of:
a1) engaging pipette tips of a first type which are held in a rack in a first position with a first process head;
a2) transferring said fluid sample from a sample vessel to a processing vessel with said pipette tips of a first type engaged to said first process head;
a3) placing said pipette tips in said rack and disengaging said pipette tips from said process head;
a4) transporting said rack comprising said pipette tips and said processing vessel to a second position; and
a5) engaging said pipette tips of a first type which are held in said rack with a second process head in said second position;
b) combining together a solid support material and said fluid sample in a well of said processing vessel for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
c) isolating the solid support material from other material present in the fluid sample in a separation station; and
d) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the solid support material one or more times with a wash buffer;
wherein said pipette tip of a first type used in steps a1)-a5) is re-used after steps a1)-a5), and wherein said pipette tip of a first type is stored in said rack comprising pipette tips of a first type and pipette tips of a second type.
2. The method of claim 1 , wherein said analyte is a nucleic acid.
3. The method of claim 1 , wherein said transporting of said rack comprising said pipette tips and said processing vessel to a second position occurs between a separate first cell of an analytical instrument and a separate second cell of said analytical system.
4. The method of claim 1 , wherein said rack comprises independent chambers to accommodate pipette tips.
5. The method of claim 1 , wherein said first type of pipette tips is re-used for the washing in step d).