Bispecific anti-IGF-1R and anti-ErbB3 antibodies
Provided are bispecific antibodies that are useful as anti-neoplastic agents and that bind specifically to human IGF-1R and human ErbB3. Exemplary antibodies inhibit signal transduction through either or both of IGF-1R and ErbB3.
1. Polyvalent bispecific antibody P4−G1−M1.3 having two pairs of polypeptide chains, each pair of said two pairs comprising a heavy chain joined to a light chain by at least one heavy-light chain bond, wherein each light chain comprises the amino acid sequence set forth in SEQ ID NO:204 and each heavy chain comprises the amino acid sequence set forth in SEQ ID NO:226.
2. The polyvalent bispecific antibody of claim 1 , wherein at least one of said at least one bonds is a disulfide bond.
3. The polyvalent bispecific antibody of claim 1 in a pharmaceutically acceptable carrier suitable for injection or infusion.
4. A method for treating a subject having cancer, said method comprising administering to the subject a therapeutically effective amount of the polyvalent bispecific antibody of claim 3 .
5. A nucleic acid molecule encoding the heavy chain of the polyvalent bispecific antibody of claim 1 .
6. Polyvalent bispecific antibody P4−G1−C8 having two pairs of polypeptide chains, each pair of said two pairs comprising a heavy chain joined to a light chain by at least one heavy-light chain bond, wherein each light chain comprises the amino acid sequence set forth in SEQ ID NO:204 and each heavy chain comprises the amino acid sequence set forth in SEQ ID NO:222.
7. The polyvalent bispecific antibody of claim 6 , wherein at least one of said at least one bonds is a disulfide bond.
8. The polyvalent bispecific antibody of claim 6 in a pharmaceutically acceptable carrier suitable for injection or infusion.
9. A method for treating a subject having cancer, said method comprising administering to the subject a therapeutically effective amount of the polyvalent bispecific antibody of claim 8 .
10. A nucleic acid molecule encoding the heavy chain of the polyvalent bispecific antibody of claim 6 .
11. Polyvalent bispecific antibody M7−G1−M78 having two pairs of polypeptide chains, each pair of said two pairs comprising a heavy chain joined to a light chain by at least one heavy-light chain bond, wherein each light chain comprises the amino acid sequence set forth in SEQ ID NO:262 and each heavy chain comprises the amino acid sequence set forth in SEQ ID NO:284.
12. The polyvalent bispecific antibody of claim 11 , wherein at least one of said at least one bonds is a disulfide bond.
13. The polyvalent bispecific antibody of claim 11 in a pharmaceutically acceptable carrier suitable for injection or infusion.
14. A method for treating a subject having cancer, said method comprising administering to the subject a therapeutically effective amount of the polyvalent bispecific antibody of claim 13 .
15. A nucleic acid molecule encoding the heavy chain of the polyvalent bispecific antibody of claim 11 .