Anti-epidermal growth factor receptor antibodies and uses thereof
The present invention discloses humanized anti-epidermal growth factor receptor antibodies, which have favorable binding activity (the binding affinity being 5.09×10 −10 mol/L) and are able to inhibit the growth and migration of tumor cells. The present invention also discloses the preparation methods and uses of the antibodies.
1. An antibody, wherein the heavy chain variable region of the antibody has the amino acid sequence as set forth by SEQ ID NO: 3, and the light chain of the antibody has the amino acid sequence as set forth by SEQ ID NO: 2.
2. The antibody according to claim 1 , wherein the antibody comprises a heavy chain and the light chain, and the heavy chain comprises the heavy chain variable region and a heavy chain constant region.
3. The antibody according to claim 2 , wherein the heavy chain constant region comprises an amino acid sequence as set forth by SEQ ID NO: 11.
4. An inhibitor for inhibiting signal transduction pathway of the epidermal growth factor receptor or a tumor cell invasion, wherein the active ingredient of the inhibitor is the antibody of claim 1 .
5. The inhibitor according to claim 4 , wherein the tumor is a colon cancer.
6. The inhibitor according to claim 5 , wherein the tumor cell is a SW480 cell.
7. A drug for treating a tumor, wherein the active ingredient of the drug is the antibody of claim 1 .
8. The drug according claim 7 , wherein the tumor is a colon cancer.
9. The drug according claim 8 , wherein the tumor cell is a SW480 cell.
10. A method for treating a tumor comprising administering a therapeutically effective amount of preparing a drug comprising the antibody of claim 1 .
11. The method according to claim 10 , wherein the tumor is a colon cancer.
12. The method according to claim 11 , wherein the tumor cell is a SW480 cell.
13. A method of inhibiting signal transduction pathway of epidermal growth factor receptor comprising administering a therapeutically effective amount of the antibody of claim 1 to an individual in need thereof.
14. A method of inhibiting a tumor cell invasion comprising administering a therapeutically effective amount of the antibody of claim 1 to an individual in need thereof.
15. The method according to claim 14 , wherein the tumor is a colon cancer.
16. A method of treating a tumor comprising administering a therapeutically effective amount of the antibody of claim 1 to an individual in need thereof.
17. The method according to claim 16 , wherein the tumor is a colon cancer.
18. The method according to claim 15 , wherein the tumor cell is a SW480 cell.
19. The method according to claim 17 , wherein the tumor cell is a SW480 cell.