Smart hydrogel particles for biomarker harvesting
Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
1. A capture particle for isolating an analyte from a mixture, comprising:
a co-polymeric matrix comprising a structural monomer and an affinity monomer;
wherein the polymeric matrix has a pore size that under certain conditions allows for the analyte to enter the polymeric matrix while excluding other compounds from the mixture from entering the polymeric matrix;
wherein the analyte is selected from the group consisting of: metabolites, proteins, RNA, micro RNA, DNA, glycoproteins, lipids, glycolipids, proteolipids, hormones, cytokines, growth factors, biomarkers, drug compounds, synthetic organic compounds, volatile odorants, toxicants and pollutants; and
wherein the structural monomer is Nisopropylacrylic acid.
2. A capture particle for isolating an analyte from a mixture, comprising:
a co-polymeric matrix comprising a structural monomer and an affinity monomer;
wherein the polymeric matrix has a pore size that under certain conditions allows for the analyte to enter the polymeric matrix while excluding other compounds from the mixture from entering the polymeric matrix;
wherein the analyte is selected from the group consisting of: metabolites, proteins, RNA, micro RNA, DNA, glycoproteins, lipids, glycolipids, proteolipids, hormones, cytokines, growth factors, biomarkers, drug compounds, synthetic organic compounds, volatile odorants, toxicants and pollutants; and
wherein the affinity monomer is selected from the group consisting of: affinity dyes, boronic acid groups, nucleic acids, glycopeptides, glycoproteins, cyclodextrins, calixarenes, porphyrin groups, and aliphatic groups.