Detection of target analytes using particles and electrodes
The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.
1. A composition comprising:
a) a magnetic particle comprising a first binding ligand that specifically binds a target analyte; and
b) a colloidal particle comprising:
i) a second binding ligand that specifically binds said target analyte; and
ii) a third binding ligand different from said second binding ligand,
wherein said first and second binding ligands are antibodies, and said third binding ligand is a nucleic acid.
2. The composition according to claim 1 wherein said composition further comprises an array comprising a fourth binding ligand.
3. The composition according to claim 1 wherein said colloidal particle comprises Au.
4. A kit comprising the composition of claim 1 .