Somatostatin analogs with inhibitory activity to growth hormone release

1. A method of visualizing malignant cells having somatostatin receptor-2 (SST2) and/or somatostatin receptor-5 (SST5) that bind a somatostatin analog in a subject comprising administering to the subject a compound selected from any one of 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:1); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:2); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Asp-NH 2 (SEQ ID NO:3); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Thr-NH 2 (SEQ ID NO:4); 4-amino-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:5); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:6); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:7); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:8); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:9); and D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:10), wherein the compound is labeled with a detectable label, and visualizing malignant cells that bind the compound.

2. The method of claim 1 , wherein the compound comprises a di- or polyiodinated aromatic modification of a Tyr at position 3 of SEQ ID NOs:1-10.

3. The method of claim 1 , wherein a radioactive element is linked to the compound.

4. The compound of claim 3 , wherein the radioactive element is selected from the group consisting of scandium-47, copper-67, gallium-72, yttrium-90, iodine-125, iodine-131, samarium-153, gadolinium-159, dysprosium-165, holmium-166, ytterbium-175, lutetium-177, rhenium-186, rhenium-188, astatine-211 and bismuth-212.

5. A method of treating a somatostatin receptor-2 (SST2) and/or somatostatin receptor-5 (SST5) cell proliferative disorder in a subject comprising administering to the subject an effective amount of a compound selected from any one of 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:1); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:2); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Asp-NH 2 (SEQ ID NO:3); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Thr-NH 2 (SEQ ID NO:4); 4-amino-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:5); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:6); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:7); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:8); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:9); and D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:10) and evaluating the subject after treatment with the compound.

6. A method as in claim 5 , wherein the cell proliferative disorder is a tumor, an acromegaly, and/or a diabetes.

7. The method of claim 5 , wherein the compound comprises a di- or polyiodinated aromatic modification of a Tyr at position 3 of SEQ ID NOs:1-10.

8. The method of claim 5 , wherein a radioactive element is linked to the compound.

9. The compound of claim 8 , wherein the radioactive element is selected from the group consisting of scandium-47, copper-67, gallium-72, yttrium-90, iodine-125, iodine-131, samarium-153, gadolinium-159, dysprosium-165, holmium-166, ytterbium-175, lutetium-177, rhenium-186, rhenium-188, astatine-211 and bismuth-212.

10. A method of promoting somatostatin receptor-2 (SST2) and/or somatostatin receptor-5 (SST5) receptor activity in a mammal in need thereof comprising administering to said mammal an effective amount of a compound selected from any one of 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:1); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:2); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Asp-NH 2 (SEQ ID NO:3); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Thr-NH 2 (SEQ ID NO:4); 4-amino-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:5); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:6); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:7); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:8); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:9); and D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:10) or a pharmaceutically acceptable salt thereof wherein the activity of the receptor is increased relative to the absence of the compound.

11. A method of treating prolactin-secreting adenomas, restenosis, diabetes mellitus, hyperlipidemia, insulin insensitivity, Syndrome X, angiopathy, proliferative retinopathy, dawn phenomenon, nephropathy, gastric acid secretion, peptic ulcers, enterocutaneous and pancreaticocutaneous fistula, irritable bowel syndrome, Dumping syndrome, watery diarrhea syndrome, AIDS-related diarrhea, chemotherapy-induced diarrhea, acute or chronic pancreatitis, gastrointestinal hormone-secreting tumors, cancer, hepatoma, angiogenesis, arthritis, chronic allograft rejection, angioplasty, graft vessel bleeding or gastrointestinal bleeding, in a mammal in need thereof, which comprises administering to said mammal an effective amount of a compound selected from any one of 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:1); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:2); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Asp-NH 2 (SEQ ID NO:3); 4-amino-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-D-Thr-NH 2 (SEQ ID NO:4); 4-amino-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:5); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:6); 4-amino-3-iodo-D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:7); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Thr-NH 2 (SEQ ID NO:8); 4-amino-3-iodo-D-Phe-c[Cys-(3-iodo)-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:9); and D-Phe-c[Cys-Tyr-D-Trp-Lys-Val-Cys]-Asp-NH 2 (SEQ ID NO:10) or a pharmaceutically acceptable salt thereof and evaluating the subject after treatment with the compound.