Soluble complexes of curcumin
A composition comprising a water-soluble and stable complex formed by an alkyl ether derivative of gamma-cyclodextrin and curcumin and optionally comprising non-complexed cyclodextrin, the molar ratio of curcumin to cyclodextrin being between 1:1 and 1:6, and a method of manufacturing such a composition. The water-soluble and stable complex of curcumin is useful in therapy, e.g. for treatment of cancer, leukemia, myocardial infarction, stroke, sepsis, acute lung injury, acute liver failure, acute tubular necrosis, acute pancreatitis, radiation injury and other life-threatening conditions in a human or animal subject, as well as for preserving human or animal organs, tissues or cells at a hypothermic temperature.
1. A method of producing a water-soluble complex of curcumin with an alkyl or ether derivative of gamma-cyclodextrin comprising:
a) contacting curcumin with the gamma-cyclodextrin derivative at a molar ratio of about 1:1 to 1:6 in an aqueous phase at a pH of at least 11 to form a solution, and
b) lowering the pH of the solution to below pH 8,
whereby a stable cyclodextrin complex of curcumin is formed in said aqueous phase.
2. The method according to claim 1 , wherein the pH of the solution is lowered to a value in the range from about 3 to below 8.
3. The method according to claim 1 , comprising recovering the solution
obtained containing a cyclodextrin complex of curcumin.
4. The method according to claim 3 , wherein the solution recovered exhibits a red color at pH 7.4.
5. A method of manufacturing an endotoxin-free cyclodextrin complex of curcumin comprising:
a) contacting curcumin with the cyclodextrin in an aqueous phase at a pH of at least 11 to form a solution of curcumin and cyclodextrin,
b) lowering the pH of said solution to below pH 8, and
c) recovering the solution thus obtained containing an endotoxin-free cyclodextrin complex of curcumin.
6. The method according to claim 1 , wherein the pH of the solution is lowered to a value in the range of from about 4.0 to 7.6.