Compositions and methods for separating, characterizing and administering soluble selenoglycoproteins
The invention relates to soluble selenium compositions and methods of production, separation and purification thereof. In particular the present invention provides methods of preparing water soluble selenoglycoproteins (e.g., via extracting selenoglycoproteins from selenium enriched yeast), methods of supplementing a selenium deficient composition via admixing water soluble selenoglycoproteins with the selenium deficient composition, compositions comprising the water soluble selenoglycoproteins and methods of administering the same.
1. A composition comprising selenoglycoproteins, wherein the selenoglycoproteins contain one or more isolated fractions of acid-soluble selenoglycoproteins present in a liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast obtained from acidic hydrolysis of the selenium enriched yeast.
2. The composition of claim 1 , wherein the one or more isolated fractions of acid-soluble selenoglycoproteins are obtained, subsequent to acidic hydrolysis of the selenium enriched yeast, via pH dependent precipitation of the selenoglycoproteins.
3. The composition of claim 2 , wherein pH dependent precipitation comprises raising the pH to a level at which a portion of the selenoglycoproteins precipitate out of the liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast soluble under acidic conditions and collecting the precipitated selenoglycoproteins.
4. The composition of claim 3 , wherein the pH level is raised to 4.0.
5. The composition of claim 3 , wherein the pH level is raised to 6.0.
6. The composition of claim 1 , wherein the selenoglycoproteins contain a single fraction of selenoglycoproteins recovered from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.
7. The composition of claim 6 , wherein the single fraction of selenoglycoproteins precipitates at pH 3.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.
8. The composition of claim 6 , wherein the single fraction of selenoglycoproteins precipitates at pH 4.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.
9. The composition of claim 6 , wherein the single fraction of selenoglycoproteins precipitates at pH 6.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.
10. The composition of claim 1 , wherein the selenoglycoproteins contain two separate fractions of selenoglycoproteins recovered from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.
11. A composition comprising selenoglycoproteins and a carrier, wherein the selenoglycoproteins contain one or more isolated fractions of acid-soluble selenoglycoproteins present in a liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast obtained from acidic hydrolysis of the selenium enriched yeast.
12. The composition of claim 11 , wherein the carrier is selected from the group consisting of a polymersome, a slow-release polymer, a nanocapsule, and a molecularly imprinted polymer.
13. The composition of claim 11 , wherein the carrier is a slow-release polymer.
14. The composition of claim 11 , wherein the carrier is a polymersome used to encapsulate selenoglycoprotein.
15. The composition of claim 14 , wherein the polymersome comprises poly(ethylene oxide) (PEO) block copolymer.
16. The composition of claim 14 , wherein the polymersome comprises poly(ε-caprolactone) (PCL) diblock copolymer.
17. The composition of claim 14 , wherein the polymersome comprises poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) based diblock copolymers.
18. The composition of claim 14 , wherein the polymersome is derived from the coupling of poly(lactic acid), poly(glycolide), poly(lactic-coglycolic acid) or poly(3-hydroxybutyrate) with PEO.
19. The composition of claim 14 , wherein the average diameter of a polymersome encapsulating selenoglycoprotein is 50-300 nm.