Markers of definitive endoderm
Disclosed herein are reagent-cell complexes comprising one or more definitive endoderm cells. Also described herein are compositions for detecting definitive endoderm. Method of enriching, isolating and/or purifying definitive endoderm cells are also disclosed.
1. An isolated human cell population wherein at least 20% of cells in said cell population form an ex vivo reagent-cell complex comprising:
a human definitive endoderm cell expressing a marker selected from the group consisting of CALCR, CMKOR1, CXCR4, GPR37, NTN4, RTN4RL1, SLC5A9, SLC40A1, and TRPA1, said definitive endoderm cell being a multipotent cell that can differentiate into cells of the gut tube or organs derived therefrom; and
a reagent bound to said marker.
2. The reagent cell complex of claim 1 , wherein said marker comprises a polypeptide selected from the group consisting of CXCR4, GPR37, NTN4, RTN4RL1, SLC40A1, SLC5A9 and TRPA1.
3. The human cell population of claim 1 , wherein at least 40% of said human cells form said ex vivo reagent-cell complex.
4. The human cell population of claim 1 , wherein at least 60% of said human cells form said ex vivo reagent-cell complex.
5. The human cell population of claim 1 , wherein at least 80% of said human cells form said ex vivo reagent-cell complex.
6. An isolated human cell population wherein at least 20% of cells in said cell population form an ex vivo reagent-cell complex comprising:
a human definitive endoderm cell expressing CXCR4, said definitive endoderm cell being a multipotent cell that can differentiate into cells of the gut tube or organs derived therefrom and a reagent bound to CXCR4.
7. The human cell population of claim 6 , wherein at least 40% of said human cells form said ex vivo reagent-cell complex complexes.
8. The human cell population of claim 6 , wherein at least 60% of said human cells form said ex vivo reagent-cell complex.
9. The human cell population of claim 6 , wherein at least 80% of said human cells form said ex vivo reagent-cell complex.