Antibody binding to lysophosphatidylglucoside, and composition comprising the same
In order to identify a factor demonstrating a repellent effect on axon extension of a neuron, lysophosphatidylglucoside (LPG) has been found to have an activity as a chemorepellent effect on axon guidance of a DRG sensory neuron specific to a neuron expressing TrkA, an NGF receptor. In addition, it has been found out that suppressing the function of LPG makes it possible to suppress a repellent effect of LPG on axon extension of a neuron expressing TrkA. A molecule capable of suppressing the function of LPG can be a composition promoting repairing of a neural circuit in neuronal damages and the like.
1. An antibody comprising: a light chain variable region comprising the complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NOs: 1 to 3; and a heavy chain variable region comprising the complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NOs: 4 to 6.
2. An antibody comprising: a light chain variable region comprising the amino acid sequence of SEQ ID NO: 7 or the amino acid sequence of SEQ ID NO: 7 in which one to 10 amino acids in the framework region are substituted, deleted, added, and/or inserted; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 8 or the amino acid sequence of SEQ ID NO: 8 in which one to 10 amino acids in the framework region are substituted, deleted, added, and/or inserted.
3. An antibody comprising: a light chain variable region comprising the complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NOs: 11 to 13; and a heavy chain variable region comprising the complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NOs: 14 to 16.
4. An antibody comprising: a light chain variable region comprising the amino acid sequence of SEQ ID NO: 17 or the amino acid sequence of SEQ ID NO: 17 in which one to 10 amino acids in the framework region are substituted, deleted, added, and/or inserted; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 18 or the amino acid sequence of SEQ ID NO: 18 in which one to 10 amino acids are substituted, deleted, added, and/or inserted.
5. A composition for suppressing a repellent effect of lysophosphatidylglucoside on axon extension of a neuron expressing TrkA, the composition comprising the antibody of claim 1 , 2 , 3 or 4 as an active ingredient.
6. The composition of claim 5 , which is a pharmaceutical composition.
7. A method for extending an axon of a neuron expressing TrkA, the method comprising administering the antibody of claim 1 , 2 , 3 or 4 to a subject in need thereof to suppress a repellent effect of lysophosphatidylglucoside.