Cancer cell targeting using nanoparticles
The present invention generally relates to polymers and macromolecules, in particular, to polymers useful in particles such as nanoparticles. One aspect of the invention is directed to a method of developing nanoparticles with desired properties. In one set of embodiments, the method includes producing libraries of nanoparticles having highly controlled properties, which can be formed by mixing together two or more macromolecules in different ratios. One or more of the macromolecules may be a polymeric conjugate of a moiety to a biocompatible polymer. In some cases, the nanoparticle may contain a drug. Other aspects of the invention are directed to methods using nanoparticle libraries.
1. A nanoparticle comprising:
(a) an anticancer agent;
(b) a diblock copolymer of (i) poly(ethylene glycol) and (ii) PLA or PLGA; and
(c) a prostate specific membrane antigen ligand conjugated to a diblock copolymer of (i) poly(ethylene glycol) and (ii) PLA or PLGA; wherein the prostate specific membrane antigen ligand has the structure
2. The nanoparticle of claim 1 , having a diameter in the range of 80 nm to 200 nm.
3. The nanoparticle of claim 1 , wherein the anticancer agent is paclitaxel, docetaxel, mitoxandrone, doxorubicin, gemcitabine, 5-fluorouracil, daunorubicin or 9-dihydrotaxol.
4. The nanoparticle of claim 3 , wherein the anticancer agent is paclitaxel, docetaxel or 9-dihydrotaxol.
5. The nanoparticle of claim 4 , wherein the anticancer agent is paclitaxel.
6. The nanoparticle of claim 4 , wherein the anticancer agent is docetaxel.
7. A pharmaceutical composition comprising:
(a) a plurality of nanoparticles, wherein each nanoparticle comprises:
an anticancer agent;
a diblock copolymer of (i) poly(ethylene glycol) and (ii) PLA or PLGA; and
a prostate specific membrane antigen ligand conjugated to a diblock copolymer of poly(ethylene glycol) and polylactic acid, wherein the prostate specific membrane antigen ligand has the structure
and
(b) a pharmaceutically acceptable carrier.
8. The pharmaceutical composition of claim 7 , wherein the nanoparticles have a diameter of 80 nm to 200 nm.
9. The pharmaceutical composition of claim 7 , wherein the anticancer agent is paclitaxel, docetaxel, mitoxandrone, doxorubicin, gemcitabine, 5-fluorouracil, daunorubicin or 9-dihydrotaxol.
10. The pharmaceutical composition of claim 7 , wherein the anticancer agent is paclitaxel, docetaxel or 9-dihydrotaxol.
11. The pharmaceutical composition of claim 7 , wherein the anticancer agent is paclitaxel.
12. The pharmaceutical composition of claim 7 , wherein the anticancer agent is docetaxel.