Mapc generation of lung tissue
The present invention relates to methods of improving function in lung tissue by administering a population of multipotent adult progenitor cells (“MAPCs”) or differentiated progeny thereof.
1. A method to produce alveolar type II epithelial cells (1) in vitro by exposing cells (2) in vitro to factors that result in differentiation of said cells (2) to cells with an alveolar type II epithelial phenotype (1), said cells (2) being isolated expanded human multipotent non-embryonic non-germ cells that can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages and express telomerase, said cells have undergone at least 10-40 cell doublings in culture, so as to produce cells with an alveolar type II epithelial phenotype (1).
2. The method of claim 1 further comprising isolating the differentiated alveolar type II epithelial cells.
3. The method of claim 1 further comprising growing the alveolar type II epithelial cells in culture medium.
4. The method of any of claims 1 - 3 further comprising mixing an effective therapeutic amount of the alveolar type II epithelial cells with a pharmaceutically acceptable carrier.