Cardiac stem cell and myocyte secreted paracrine factors
The invention relates to secreted proteins from cardiac stem cells (cardiospheres and cardiosphere-derived cells) or myocytes for diagnostic and/or therapeutic use.
1. A method for screening a cardiac patient as a candidate for stem cell therapy, said method comprising measuring in a biological sample from the patient the amount of apolipoprotein E precursor, atriopeptigen (ANP, ANF), adrenomedullin, brain acid soluble protein 1 (BASP 1 protein), cathepsin B precursor, collagen alpha-1 (VI) chain precursor, connective tissue growth factor, cystatin E/M, Dkk3 protein, insulin-like growth factor-binding protein 2 precursor, interleukin-1 receptor-like 1 precursor (ST2), matrix metallopeptidase 2, metalloproteinase inhibitor 1 precursor (TIMP-1), metalloproteinase inhibitor 2 precursor (TIMP-2), mimecan precursor, procollagen type III alpha 1, procollagen type V alpha 1 and tropoelastin, comparing said measurement to a baseline value, wherein a statistically significant amount of the protein(s) compared to the baseline value indicates that the patient would be likely to benefit from stem cell therapy.
2. The method of claim 1 , wherein the statistically significant amount represents at least a 5% increase over baseline value.
3. The method of claim 1 , wherein the cardiac patient is suffering from angina, acute myocardial injury, cellular necrosis and myocardium hypertrophy or heart failure.
4. The method of claim 1 , wherein the biological sample is a whole blood sample, serum sample, plasma sample, tissue biopsy sample, a sample of cultured cells derived from tissue, or a sample of circulating stem cells such as EPC.