Methods of using capped mesoporous silicates
The invention provides an article having a mesoporous silicate matrix, such as a particle, having one or more pores; and one or more releasable caps obstructing one or more of the pores for delivery of one or more agents to plant cells or other chlorophyll containing cells, or fungi.
1. A method for delivering more than one agent to a plant or fungal cell, comprising:
contacting plant or fungal cells having a cell wall with an amount of a composition comprising a mesoporous silicate body having i) one or more pores which have one or more removable gold nanoparticle caps obstructing the one or more pores and a first agent in the one or more pores, which removable gold nanoparticle caps allow for controlled release of the first agent from the one or more pores and ii) a second agent coated on the surface of the silicate body, so as to deliver the first and second agents into the cells.
2. The method of claim 1 wherein the coating comprises a polymer.
3. The method of claim 2 wherein the polymer is TEG.
4. The method of claim 1 wherein the coating comprises a dendrimer.
5. The method of claim 2 wherein the polymer comprises a poly(amidoamine).
6. The method of claim 1 wherein the second agent comprises isolated nucleic acid encoding at least one gene product.
7. The method of claim 6 wherein the isolated nucleic acid comprises DNA.
8. The method of claim 7 wherein the isolated DNA is on a plasmid.
9. The method of claim 1 wherein the mesoporous silicate body is a particle from about 1 nm to about 10 microns.
10. The method of claim 1 wherein the pores are about 1 to 50 nm in diameter.
11. The method of claim 1 wherein the pores are about 1 nm to about 3 nm in diameter.
12. The method of claim 1 wherein the mesoporous silicate body is a sphere.
13. The method of claim 12 wherein the mesoporous silicate body is a sphere having a diameter of about 100 to about 300 nm.
14. The method of claim 1 wherein the mesoporous silicate body is a rod.
15. The method of claim 1 wherein the one or more caps are covalently bonded to the mesoporous silicate body.
16. The method of claim 15 wherein the one or more caps are covalently bonded to the mesoporous silicate through a linking group that comprises disulfide.
17. The method of claim 1 wherein the one or more caps are removed by contacting the mesoporous silicate body with a reducing agent.
18. The method of claim 17 wherein the reducing agent is dithiothreitol (DTT).
19. The method of claim 1 wherein the first agent comprises a hormone, an antifungal agent, an antiviral agent, or a nutrient.
20. The method of claim 1 wherein the plant cells are dicot cells.
21. The method of claim 1 wherein the plant cells are monocot cells.
22. The method of claim 1 wherein the plant cells are protoplasts.
23. The method of claim 1 wherein the composition is contacted with a plant.
24. The method of claim 1 wherein the composition is contacted with the plant cells via bombardment.