Apparatus for securely processing biological sample
An apparatus for securely processing biological sample is used to wash, separate, or purify biological molecules, such as DNAs, RNAs, and proteins. The apparatus comprises at least one semi-permeable membrane column, a vacuum manifold, and at least one optional adaptor column. The semi-permeable membrane column is loosely received in the slot of the vacuum manifold. When the adaptor column is used, the semi-permeable membrane column is loosely received in the adaptor column, and the adaptor column is loosely received in the slot of the vacuum manifold. The apparatus also comprises sealing elements which are inserted between the column and the slot. When the semi-permeable membrane column contains liquid sample and vacuum is applied to the vacuum manifold, the liquid in the semi-permeable membrane column can be drawn out of the column through the membrane.
1. An apparatus for securely processing biological sample, comprising:
at least one semi-permeable column, whereby the inner portion of which defines a first receiving space, the bottom of the first receiving space has at least one semi-permeable membrane, the top portion of the semi-permeable column has a first opening and a flange extending radially and outwardly from the first opening, and the bottom of the semi-permeable column has a first outlet;
at least one adaptor column, whereby the interior of which defines a second receiving space, the top portion of which has a second opening and a second flange extending radially and outwardly from the second opening, the bottom portion of which has a second outlet protruding downwardly, the diameter of the second receiving space is merely slightly larger than the outer diameter of the semi-permeable column thereby allowing the semi-permeable column to be secured stably during operation, the second flange having a second sealing element fitting therearound such that when the semi-permeable is inserted into the adaptor column, the first flange will rest on the second sealing element;
a vacuum manifold, comprising:
a base, the interior of which defines a receiving space; and
a lid covering the base, comprising at least one slot, whereby the internal diameter of which is merely slightly larger than the outer diameter of the adaptor column thereby allowing the adaptor column to be secured stably during operation, and the slot forms a through hole at the bottom thereof for communicating with the receiving space of the base;
at least one sealing element positioned around the adaptor column and in the gap formed between the slot and the adaptor column such that when the adaptor column is inserted into the slot, it can be secured in position, and when vacuum is applied to the vacuum manifold, air would not pass through between the adaptor column and the sealing element, nor through between the first flange and the second sealing element.