Compositions and methods for conversion of lignocellulosic material to fermentable sugars and products produced therefrom
The present invention relates to compositions and methods for the conversion of lignocellulosic material to fermentable sugars and to products produced therefrom (e.g., ethanol, foodstuffs, etc.). In particular, the invention provides lignocellulose-degrading compositions (e.g., generated via incubation of microbes with lignocellulosic priming feedstock in solid-state fermentation format) and methods of using the same (e.g., in saccharification and/or hydrolysis steps (e.g., on ethanologenic feedstock) and as food or feed additives).
1. A composition for generation of ethanol from a lignocellulosic feedstock comprising a lignocellulosic feedstock degradation composition, wherein the lignocellulosic feedstock degradation composition comprises:
a) a lignocellulosic priming feedstock; wherein the lignocellulosic priming feedstock comprises dried distiller's grains (DDG) or dried distiller's grains with solubles (DDGS); and
b) one or more lignocellulose-degrading microbes, wherein the one or more lignocellulose-degrading microbes are selected from the group consisting of Aspergillus niger, Aspergillus oryzae , and Rhizopus oligosporus;
wherein the lignocellulose feedstock degradation composition is generated via incubation of the lignocellulosic priming feedstock with the one or more lignocellulose-degrading microbes under solid state fermentation conditions; and
wherein the lignocellulosic feedstock degradation composition, when added to an ethanologenic fermentation of lignocellulosic feedstock, increases the yield of ethanol obtained from the ethanologenic fermentation by at least 9% compared to the yield of ethanol from ethnologenic fermentation of the lignocellulosic feedstock in the absence of the lignocellulosic feedstock degradation composition.
2. The composition of claim 1 , wherein the Rhizopus oligosporus is Rhizopus oligosporus strain 2UV3.
3. The composition of claim 1 , wherein said lignocellulosic feedstock is selected from the group consisting of forest residue, mill waste, urban wood waste, agricultural residues, bioenergy crops, stover, quinoa, corn husks, corn cobs, corn fiber, wheat straw, milo stubble, switchgrass, deciduous wood, coniferous wood, deciduous or coniferous wood chips, deciduous or coniferous sawdust, citrus waste, urban green waste or residue, food manufacturing industry waste or residue, cereal manufacturing waste or residue, hay, straw, rice straw, sugarcane, sugarcane bagasse, grain cleanings, spent brewer's grain, rice hulls, barley straw, salix, spruce, poplar, eucalyptus, Brassica carinata residue, Antigonum leptopus , sweetgum, Miscanthus, Sericea lespedeza , Chinese tallow, hemp, rapeseed, Sorghum bicolor , soybean leaves, soybeans stems, soybean pods, soybean residue, sunflower leaves, sunflower stems, seedless sunflower heads, sunflower hulls, sunflower residue, Arundo, nut shells, deciduous leaves, cotton fiber, manure, coastal Bermuda grass, clover, Johnsongrass, flax, buckwheat straw, oat straw, millet straw, amaranth straw, amaranth stems, amaranth leaves, amaranth residue, spelt straw, rye straw, alfalfa, and bamboo.
4. The composition of claim 3 , wherein said spent brewer's grain is dried distiller's grain (DDG) or dried distiller's grain with solubles (DDGS).
5. The composition of claim 1 , wherein solid state fermentation of the lignocellulosic priming feedstock with the one or more lignocellulose-degrading microbes comprises incubating the lignocellulosic priming feedstock and the one or more lignocellulose-degrading microbes at 20-40° C. for at least 5 days.
6. A method of producing ethanol from an ethanologenic feedstock comprising:
1) providing:
a) a lignocellulosic feedstock degradation composition comprising a lignocellulosic priming feedstock; wherein the lignocellulosic priming feedstock comprises dried distiller's grains (DDG) or dried distiller's grains with solubles (DDGS); and one or more lignocellulosic-degrading microbes, wherein the one or more lignocellulosic-degrading microbes are selected from the group consisting of Aspergillus niger, Aspergillus oryzae , and Rhizopus oligosporus ; wherein the lignocellulosic feedstock degradation composition is generated via incubation of the lignocellulosic priming feedstock with the one or more lignocellulosic-degrading microbes under solid state fermentation conditions; and wherein the lignocellulosic feedstock degradation composition, when added to an ethanologenic fermentation of lignocellulosic feedstock, increases the yield of ethanol obtained from the ethanologenic fermentation by at least 9% compared to the yield of ethanol from ethanologenic fermentation of the lignocellulosic feedstock in the absence of the lignocellulosic feedstock degradation composition; and
b) an ethanologenic feedstock comprising a lignocellulosic component; and
2) incubating the ethanologenic feed stock with the lignocellulosic feedstock degradation composition.
7. The method of claim 6 , wherein the ethanologenic feedstock is comprised of a feedstock selected from the group consisting of grains, storage roots, tubers, nuts, fruits, corn (maize), wheat, rice, oats, barley, rye, amaranth, buckwheat, or spelt; potato, sweet potato, taro, yam, cassava, tapioca, arrowroot, cassava, legumes, chestnut, arracacha, banana, kudzu, oca, sago, and sorghum.
8. The method of claim 7 , wherein said corn is in the form of corncobs or corn kernels.
9. A method for the production of ethanol from lignocellulosic feedstock, comprising:
i. providing a lignocellulosic feedstock degradation composition comprising a lignocellulosic priming feedstock; wherein the lignocellulosic priming feedstock comprises dried distiller's grains (DDG) or dried distiller's grains with solubles (DDGS); and one or more lignocellulosic-degrading microbes, wherein the one or more lignocellulosic-degrading microbes are selected from the group consisting of Aspergillus niger, Aspergillus oryzae , and Rhizopus oligosporus ; wherein the lignocellulosic feedstock degradation composition is generated via incubation of the lignocellulosic priming feedstock with the one or more lignocellulosic-degrading microbes under solid state fermentation conditions; and wherein the lignocellulosic feedstock degradation composition, when added to an ethanologenic fermentation of lignocellulosic feedstock, increases the yield of ethanol obtained from the ethanologenic fermentation by at least 9% compared to the yield of ethanol from ethanologenic fermentation of the lignocellulosic feedstock in the absence of the lignocellulosic feedstock degradation composition;
ii. providing an ethanologenic feedstock capable of utilization as substrate by an ethanologenic microbe in a saccharification step;
iii. mixing the lignocellulosic feedstock degradation composition with the ethanologic feedstock under conditions sufficient to promote saccharification of the ethanologenic feedstock; and
iv. conducting ethanologenic fermentation on the ethanologenic feedstock.
10. The method of claim 9 , wherein the ethanologenic feedstock is comprised of one or more starch-rich feedstock selected from among the group consisting of grains, storage roots, tubers, nuts, fruits, corn (maize), wheat, rice, oats, barley, rye, amaranth, buckwheat, or spelt; potato, sweet potato, taro, yam, cassava, tapioca, arrowroot, cassava, legumes, chestnut, arracacha, banana, kudzu, oca, sago, and sorghum.
11. The composition of claim 1 , wherein the lignocelluloseic feedstock degradation composition, when added to an ethanologenic fermentation of lignocellulosic feedstock, increases the yield of ethanol obtained from the ethanologenic fermentation by at least 20% compared to the yield of ethanol from ethnologenic fermentation of the lignocellulosic feedstock in the absence of the lignocellulosic feedstock degradation composition.