Systems and methods for bio-image calibration

1. A bio-image calibration device comprising:

a calibration portion comprising predetermined color areas;

wherein the predetermined color areas are arranged in a predetermined pattern corresponding to a watermark;

wherein the predetermined color areas are a predetermined size and a predetermined shape; and

wherein the predetermined color areas are located on one or more edges of the bio-image device.

2. The bio-image calibration device according to claim 1 , wherein said one or more edges comprise one or more of a top edge and a bottom edge of the bio-image calibration device.

3. The bio-image calibration device according to claim 1 , further comprising an aperture in the bio-image calibration device; wherein said aperture is at least partially lined with the predetermined color areas.

4. The bio-image calibration device according to claim 1 , wherein the predetermined color areas are printed on the bio-image calibration device using known color values for the following colors: cyan, magenta, yellow, and black;

wherein a color area of the predetermined color areas contains a single color selected from the group of colors consisting of cyan, magenta, yellow and black.

5. The bio-image calibration device according to claim 1 , further comprising one or more portions for labeling positioned outside of the predetermined color areas.

6. The bio-image calibration device according to claim 1 , further comprising a handle positioned outside of the predetermined color areas.

7. The bio-image calibration device according to claim 1 , wherein said bio-image calibration device is paper-based.

8. The bio-image calibration device according to claim 1 , wherein the watermark is configured to uniquely identify one or more of a company, a user, and an organization.