Immunoglobulin constant region FC receptor binding agents
IVIG replacement compounds are derived from recombinant and/or biochemical creation of immunologically active biomimetic(s). These replacement compounds are then screened in vitro to assess each replacements compound's efficiency at modulating immune function. Particular replacement compounds are selected for further in vivo validation and dosage/administration optimization. Finally, the replacement compounds are used to treat a wide range of diseases, including inflammatory and autoimmune diseases.
1. A cluster stradomer comprising two or more multimerized cluster stradomer units, wherein each of said cluster stradomer units comprises at least one multimerizing region and at least one Fc domain, wherein the multimerizing region(s) of the two or more cluster stradomer units multimerize to form the cluster stradomer, wherein the cluster stradomer is capable of specifically binding to at least two Fc receptors; wherein the at least one multimerizing region is an IgG2 hinge or an isoleucine zipper; and wherein the at least one Fc domain comprises a CH2 domain and a CH3 domain from IgG1 or IgG3 or a combination thereof.
2. The cluster stradomer of claim 1 , comprising two multimerized cluster stradomer units.
3. The cluster stradomer of claim 1 , comprising three multimerized cluster stradomer units.
4. The cluster stradomer of claim 1 , comprising four multimerized cluster stradomer units.
5. The cluster stradomer of claim 1 , comprising five multimerized cluster stradomer units.
6. The cluster stradomer of claim 1 , wherein the at least one Fc domain comprises an IgG1 hinge, an IgG1 CH2 domain, and an IgG1 CH3 domain.
7. The cluster stradomer of claim 1 , wherein the at least one Fc domain comprises an IgG3 hinge, an IgG3 CH2 domain, and an IgG3 CH3 domain.
8. The cluster stradomer of claim 1 , wherein at least one of the cluster stradomer units comprises two or more Fc domains.
9. The cluster stradomer of claim 1 , wherein each of the cluster stradomer units comprises two or more Fc domains.
10. The cluster stradomer of claim 1 , wherein each of the cluster stradomer units is capable of specifically binding to at least one FcR.
11. The cluster stradomer of claim 10 , wherein the at least one FcR is human Fcγ receptor I, human Fcγ receptor II, human Fcγ receptor III, or human Fcγ receptor IV.
12. The duster stradomer of claim 11 , wherein the human Fcγ receptor III is human Fcγ receptor IIIa.
13. The cluster stradomer of claim 1 , wherein the at least one multimerizing region is an IgG2 hinge.
14. A cluster stradomer comprising two or inure multimerized cluster stradomer units, wherein each of said cluster stradomer units comprises an IgG2 hinge multimerizing region and an IgG1 Fc domain comprising a CH2 and CH3 domain, wherein the IgG2 hinge multimerizing region of the two or more cluster stradomer units multimerize to form the cluster stradomer, and wherein the cluster stradomer is capable of specifically binding to at least two Fcγ receptors.
15. A cluster stradomer comprising two or more multimerized cluster stradomer units, wherein each of said cluster stradomer units comprises an isoleucine zipper multimerizing region and an IgG1 Fc domain comprising a CH2 and CH3 domain, wherein the isoleucine zipper multimerizing region of each of the two or more cluster stradomer units multimerize to form the cluster stradomer, and wherein the cluster stradomer is capable of specifically binding to at least two Fcγ receptors.