Methods for selecting protease resistant polypeptides
The disclosure relates to a method for selecting, isolating and/or recovering a peptide or polypeptide from a library or a repertoire of peptides and polypeptides (e.g., a display system) that is resistant to degradation by a protease such as a protease found in the serum. Generally, the method comprises providing a library or repertoire of peptides or polypeptides, incubating the library or repertoire with a protease under conditions suitable for protease activity, and selecting, isolating and/or recovering a peptide or polypeptide that is resistant to degradation by the protease and has a desired biological activity. The selected peptides and polypeptides have utility as therapeutics, e.g., for treating disease in humans.
1. An immunoglobulin single variable domain comprising a CDR1 amino acid sequence as shown at residues 24 to 34 of SEQ ID NO: 22; a CDR2 amino acid sequence as shown at residues 49 to 56 of SEQ ID NO: 22; and a CDR3 amino acid sequence as shown at residues 89 to 97 of SEQ ID NO: 22.
2. An isolated polypeptide comprising the amino acid sequence shown in SEQ ID NO: 22.
3. An isolated polypeptide comprising the amino acid sequence shown in SEQ ID NO: 21.