Methods and compositions for determining the purity of chemically synthesized nucleic acids
This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.
1. An article useful for determining incomplete deprotection of a synthetic oligonucleotide in an immunoassay, said article comprising:
a solid support having a surface portion, said surface portion having at least two separate discrete regions formed thereon;
a first oligonucleotide bound to one of said separate discrete regions, said first oligonucleotide having a protecting group bound thereto; and
a second oligonucleotide bound to another of said separate discrete regions, said second oligonucleotide not having said protecting group bound thereto;
wherein the nucleotide sequence of said first and second oligonucleotides are the same.
2. An article according to claim 1 , further comprising:
a third oligonucleotide bound to another of said separate discrete regions; said third oligonucleotide also having said protecting group bound to said first oligonucleotide bound thereto;
wherein said third oligonucleotide is partially deprotected;
and wherein the nucleotide sequence of said first, second, and third oligonucleotides are the same.
3. An article according to claim 1 , wherein said substrate comprises a nitrocellulose strip.