Production of volatile dienes by enzymatic dehydration of light alkenols
Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.
1. A method for producing 1,3-butadiene comprising enzymatically converting an alcohol compound of the general formula C 4 H 8 O to 1,3-butadiene by using an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity.
2. The method of claim 1 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
3. The method of claim 1 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
4. The method of claim 3 , wherein the alcohol compound is but-2-en-1-ol.
5. The method of claim 3 , wherein the alcohol compound is but-3-en-2-ol.
6. The method of claim 3 , wherein the alcohol compound is but-3-en-1-ol.
7. The method of claim 3 , wherein said method is carried out in vitro.
8. The method of claim 3 , wherein a microorganism produces the enzyme.
9. The method of claim 1 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
10. The method of claim 1 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.
11. The method of claim 10 , wherein said method is carried out in vitro.
12. The method of claim 10 , wherein a microorganism produces the enzyme.
13. A composition comprising a microorganism which produces an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1 and an alcohol compound of the general formula C 4 H 8 O, wherein said enzyme has alkenol dehydratase activity.
14. The composition of claim 13 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
15. The composition of claim 13 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
16. The composition of claim 15 , wherein the alcohol compound is but-2-en-1-ol.
17. The composition of claim 15 , wherein the alcohol compound is but-3-en-2-ol.
18. The composition of claim 15 , wherein the alcohol compound is but-3-en-1-ol.
19. The composition of claim 13 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
20. The composition of claim 13 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.
21. A composition comprising an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1 and an alcohol compound of the general formula C 4 H 8 O, wherein said enzyme has alkenol dehydratase activity.
22. The composition of claim 21 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
23. The composition of claim 21 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
24. The composition of claim 23 , wherein the alcohol compound is but-2-en-1-ol.
25. The composition of claim 23 , wherein the alcohol compound is but-3-en-2-ol.
26. The composition of claim 23 , wherein the alcohol compound is but-3-en-1-ol.
27. The composition of claim 21 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
28. The composition of claim 21 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.