Methods and compositions for wound healing
The present invention relates to methods and compositions for wound healing. In particular, the present invention relates to promoting and enhancing wound healing by utilizing cross-linker covalent modification molecules to attach and deliver wound active agents to a wound. In addition, the present invention provides methods and compositions utilizing oppositely charged polymers to form a polyelectrolyte layer on a wound surface. The invention further relates to incorporating wound active agents into a polyelectrolyte layer for delivery to a wound.
1. A method of treatment comprising:
a) providing a subject having a wound comprising a wound bed presenting amine and sulfhydryl groups, at least one bifunctional covalent modification agent that covalently reacts with a chemical entity selected from the group consisting of amine and sulfhydryl groups, and at least one wound active agent;
b) contacting said wound with said at least one bifunctional covalent modification agent that covalently reacts with a chemical entity selected from the group consisting of amine and sulfhydryl groups in said wound bed to provide a covalently modified wound bed, and
c) contacting said covalently modified wound bed with said at least one wound active agent under conditions such that said at least one wound active agent is incorporated into said modified wound bed by covalently binding to said bifunctional covalent modification agent and healing of said wound is enhanced, wherein said wound active agent is selected from the group consisting of trophic factors, enzymes, enzyme inhibitors, defensins, anti-infective agents, analgesics, anticoagulants, anti-inflammatory agents, vasoconstrictors, vasodilators, diuretics, and anti-cancer agents.
2. The method of claim 1 , wherein said wound active agent is applied to said covalently modified wound bed to form a gradient.
3. The method of claim 1 , wherein said bifunctional covalent modification agent is applied to said wound bed by a method selected from the group consisting of stamping, spraying, pumping, painting, smearing and printing.
4. The method of claim 1 , wherein said bifunctional covalent modification agent comprises an alkyne group.