Newly identified human rhinovirus of HRV-C and methods and kits for detecting HRV-Cs
The characterization of a new strain of human rhinovirus of genetic group C (HRV-C) as well as methods and kits for detecting the presence of HRV-C by PCR amplification are provided.
1. An isolated human rhinovirus of genetic group C (HRV-C) comprising an RNA genome that has at least 75% sequence identity with the full-length cDNA sequence of SEQ ID NO: 1, wherein thymine nucleotides in SEQ ID NO: 1 represent uracil nucleotides in the RNA genome.
2. The isolated human rhinovirus of claim 1 , wherein the RNA genome has at least 85% sequence identity with the full-length cDNA sequence of SEQ ID NO: 1.
3. The isolated human rhinovirus of claim 1 , wherein the RNA genome has at least 95% sequence identity with the full-length cDNA sequence of SEQ ID NO: 1.
4. The isolated human rhinovirus of claim 1 , wherein the RNA genome has the same sequence as the full-length cDNA sequence of SEQ ID NO: 1.
5. The isolated human rhinovirus of claim 1 , wherein the RNA genome comprises at least one reading frame encoding a polyprotein.
6. The isolated human rhinovirus of claim 5 , wherein the polyprotein has the full-length amino acid sequence of SEQ ID NO: 20.
7. An isolated DNA molecule comprising a nucleic acid sequence having at least 95% sequence identity with at least 150 consecutive nucleotides of nucleotides 627 to 7064 in SEQ ID NO: 1 or with at least 150 consecutive nucleotides of the sequence complementary to nucleotides 627 to 7064 in SEQ ID NO: 1.
8. The isolated DNA molecule of claim 7 , wherein the nucleic acid sequence has at least 95% sequence identity with a full-length sequence selected from the group consisting of the full-length sequences of SEQ ID NOS: 21-23 and the sequences fully complementary thereto.
9. An isolated polypeptide produced by the rhinovirus of claim 1 .
10. An isolated polypeptide encoded by the DNA of claim 7 .
11. An isolated polypeptide comprising the full-length amino acid sequence of SEQ ID NO: 20.
12. A kit for diagnosing infection by a human rhinovirus of genetic group C (HRV-C) strain, the kit comprising a container containing the polypeptide of claim 10 .
13. A method for detecting the presence of a human rhinovirus of genetic group C (HRV-C) strain, comprising:
contacting a sample with the polypeptide of claim 10 ; and
detecting whether an immune complex between the polypeptide and anti-HRV-C antibodies is formed,
wherein the presence of the immune complex indicates the presence of the HRV-C strain.
14. The method of claim 13 , wherein the sample is selected from the group consisting of human nasal samples, human oral samples, and culture supernatants.