Paper based spectrophotometric detection of blood hemoglobin concentration
The present disclosure relates to the use of a paper medium to measure blood hemoglobin concentration. In certain embodiments, spectrophotometric techniques are used to measure light transmission at specified wavelengths through a paper medium containing a blood sample. The light transmission information is then used in the calculation of blood hemoglobin concentration. In certain embodiments, the paper medium may be chemically treated to lyse the blood sample prior to measurement of the light transmission information.
1. One or more non-transitory, machine readable media encoding routines that, when executed by a processor, cause acts to be performed comprising:
receiving a first input indicative of absorbance of light at a first wavelength through a blood sample on a paper medium;
receiving a second input indicative of absorbance of light at a second wavelength through the blood sample on the paper medium;
correcting for baseline intensity in the first input based on the second input to generate a normalized measure of absorbance by hemoglobin;
calculating a hemoglobin concentration using the normalized measure.
2. The one or more machine readable media of claim 1 , wherein the hematocrit is calculated using an empirically determined correlation or constant relating optical density and hemoglobin concentration.
3. The one or more machine readable media of claim 1 , wherein the first wavelength of light is about 590 nm.
4. The one or more machine readable media of claim 1 , wherein the second wavelength of light is about 730 nm.
5. The one or more machine readable media of claim 1 , wherein correcting for baseline intensity in the first input comprises subtracting the second input from the first input.
6. The one or more machine readable media of claim 1 , wherein the first wavelength of light constitutes an isosbestic point for oxyhemoglobin and deoxyhemoglobin.
7. A method for measuring hemoglobin concentration, comprising the acts of:
measuring the absorption of light by a blood sample on a paper medium at two wavelengths;
subtracting a value representative of the absorption of light at the second wavelength from a value representative of the absorption of light at the first wavelength to derive a normalized absorption value; and
calculating a hemoglobin concentration based on the normalized absorption value using an empirically determined correlation or constant relating optical density and hemoglobin concentration.
8. The method of claim 7 , wherein the blood sample comprises lysed blood cells.
9. The method of claim 7 , wherein the blood sample is lysed on the paper medium prior to the absorption of light being measured.