Continuous polymer synthesizer
Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.
1. A method for synthesizing molecules, comprising:
(a) providing a control system and a stage comprising a plurality of reaction sites in a fixed order and at fixed distances from one another,
(b) providing a plurality of dispensing stations connected to the control system and configured to deliver reagents to said reaction sites to start a reaction, wherein said dispensing stations are located along said stage at fixed distances from one another and wherein said control system correlates said fixed distances to the duration for said reaction thereby providing a schedule for rotating the stage; and
(c) rotating the stage and sequentially dispensing said reagents into said reaction sites on said schedule so that a first reaction in a reaction site is completed before a second reaction in said reaction site is initiated; and
said reaction sites rotate from a first dispensing station to a second dispensing station during a step of said reaction, wherein said step of said reaction is completed before said reaction site is positioned below said second dispensing station.
2. The method of claim 1 , wherein said molecules comprise polymers.
3. The method of claim 2 , wherein sequentially dispensing said reagents comprises sequentially dispensing monomers to said polymers.
4. The method of claim 1 , wherein said stage comprises a circular table.
5. The method of claim 4 , wherein said dispensing stations are located along an outer circumference of said circular table.
6. The method of claim 5 , wherein said circular table rotates at a predetermined speed so that said reaction sites move below said dispensing stations at a predetermined time.
7. The method of claim 1 , wherein said molecules comprise nucleic acids.
8. The method of claim 7 , wherein sequentially dispensing said reagents comprises sequentially dispensing nucleotides to said nucleic acids.
9. The method of claim 1 , wherein said molecules comprise polypeptides.
10. The method of claim 9 , wherein sequentially dispensing said reagents comprises sequentially dispensing amino acids to said polypeptides.