Soybean transcription terminators and use in expression of transgenic genes in plants
Five novel plant transcription terminators MYB2, KTI1, PIP1, EF1A2, and MTH1 are isolated from soybean and their functions in the regulation of RNA transcription and processing in plants are described.
1. A recombinant DNA construct comprising a promoter, at least one heterologous nucleotide sequence, and an isolated polynucleotide comprising:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:3;
b) a nucleotide sequence comprising a sequence having at least 95% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a); or
c) the nucleotide sequence complementary to (a) or (b);
wherein said nucleotide sequence functions as a terminator and wherein the promoter, heterologous nucleotide sequence, and isolated polynucleotide are operably linked.
2. A recombinant DNA construct comprising a promoter, at least one heterologous nucleotide sequence, and an isolated polynucleotide comprising:
a) a nucleotide sequence comprising a fragment of SEQ ID NO:3;
b) a nucleotide sequence comprising a sequence having at least 95% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a); or
c) the nucleotide sequence complementary to (a) or (b);
wherein said nucleotide sequence functions as a terminator and wherein the promoter, heterologous nucleotide sequence, and isolated polynucleotide are operably linked.
3. A vector comprising the recombinant DNA construct of claim 1 or 2 .
4. A cell comprising the recombinant DNA construct of claim 1 or 2 .
5. The cell of claim 4 , wherein the cell is a plant cell.
6. A transgenic plant having stably incorporated into its genome the recombinant DNA construct of claim 1 or 2 .
7. The transgenic plant of claim 6 wherein said plant is a dicot.
8. The transgenic plant of claim 7 wherein said plant is soybean.
9. Transgenic seed produced by the transgenic plant of claim 7 .
10. A method of expressing a coding sequence or a functional RNA in a plant comprising:
a) introducing the recombinant DNA construct of claim 1 or 2 into the plant, wherein the at least one heterologous nucleotide sequence comprises the coding sequence or the functional RNA;
b) growing the plant of step a); and
c) selecting the plant displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.
11. A method of transgenically altering a marketable plant trait, comprising:
a) introducing the recombinant DNA construct of claim 1 or 2 into a plant cell;
b) growing a fertile, mature plant from the plant cell resulting from step a); and
c) selecting the plant expressing the at least one heterologous nucleotide sequence in at least one plant tissue based on the altered marketable trait.
12. The method of claim 11 wherein the marketable plant trait is selected from the group consisting of: disease resistance, herbicide resistance, insect resistance, carbohydrate metabolism, fatty acid metabolism, amino acid metabolism, plant development, plant growth regulation, yield improvement, drought resistance, cold resistance, heat resistance, and salt resistance.