Optimized FC variants
A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.
1. A variant of a parent polypeptide comprising a Fc region, which variant exhibits increased binding to FcRn as compared to said parent polypeptide and comprises
a combination of amino acid modifications selected from the group consisting of:
N315D/A330V/N361D/A378V/N434Y,
V264E/N315D/A378V/N390S/G420R/N434Y,
N315D/A378V/N434Y,
N315D/A330V/A378V/N434Y,
N315D/K334E/A378V/N434Y,
V264E/N315D/A378V,
P228R/N315D/A330V/N361D/A378V/N434Y,
P228R/P230S/N315D/A330V/N361D/A378V/N434Y,
P228L/N315D/A330V/N361D/A378V/N434Y,
P228L/P230S/N315D/A330V/N361D/A378V/N434Y,
P230S/N315D/A330V/N361D/A378V/N434Y, and
P230T/V264E/N315D/K370R/A378V
of the Fc region, wherein the numbering of the amino acids in the Fc region is that of the EU index as in Kabat.
2. The variant according to claim 1 , wherein said variant is an antibody.
3. The variant according to claim 2 , wherein said antibody is an IgG antibody.
4. A pharmaceutical composition comprising a variant as defined in claim 1 .
5. A medicament comprising a variant according to claim 1 .
6. An isolated nucleic acid encoding a variant as defined in claim 1 .
7. A vector comprising the nucleic acid of claim 6 .
8. An isolated host cell containing the vector of claim 7 .
9. A method for producing a variant according to claim 1 , comprising culturing a host cell containing a vector comprising an isolated nucleic acid encoding said variant so that the nucleic acid is expressed.