Method for preparing a transgenic animal of simultaneous multiple-gene expression
A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient method for the preparation of combined-gene transferred animals.
1. A nucleotide molecule comprising the sequence of SEQ ID NO: 1.
2. A method for preparing a transgenic porcine embryo, the method comprising:
introducing the nucleotide molecule of claim 1 into a target porcine embryo so as to obtain a transgenic porcine embryo, said nucleotide molecule comprising phytase gene and human myxovirus resistant gene A.
3. The method according to claim 2 , wherein the target porcine embryo is a porcine embryo in pronuclear stage.
4. A method for cultivating a transgenic pig, the method comprising:
transplanting the transgenic porcine embryo prepared by the method of claim 2 into uterine horns of estrus synchronized sow through vaginal cervix so as to obtain the transgenic pig, wherein said transgenic pig is capable of simultaneously expressing multiple genes.