Patent
The invention provides proteins from Staphylococcus aureus including amino acid sequences and the corresponding nucleotide sequences. The proteins are useful for vaccines, immunogenic compositions, diagnostics, enzymatic studies and also as targets for antibiotics.
1. A composition comprising an isolated polypeptide selected from the group consisting of:
(a) an isolated polypeptide comprising a fragment of SEQ ID NO: 2388, wherein the fragment of SEQ ID NO: 2388 includes at least twenty consecutive amino acids from SEQ ID NO: 2388; and
(b) an isolated polypeptide comprising an amino acid sequence having at least 95% sequence identity with SEQ ID NO: 2388.
2. The composition of claim 1 , wherein the isolated polypeptide is (a), and wherein the amino acid sequence is at least 99% identical to SEQ ID NO: 2388.
3. The composition of claim 1 , wherein the isolated polypeptide is (b), and wherein the fragment of SEQ ID NO: 2388 includes at least fifty consecutive amino acids from SEQ ID NO: 2388.
4. The composition of claim 1 , wherein the isolated polypeptide is (b), wherein the fragment of SEQ ID NO: 2388 includes at least one hundred consecutive amino acids from SEQ ID NO: 2388.
5. The composition of claim 1 , wherein the isolated polypeptide comprises a hybrid polypeptide represented by the formula NH 2 -A-[-X-L-] n -B-COOH, wherein X is: (a) a fragment having at least 90% sequence identity to at least twenty consecutive amino acids from SEQ ID NO: 2388, or (b) an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2388; L is an optional linker amino acid sequence; A is an optional N-terminal amino acid sequence; B is an optional C-terminal amino acid sequence; and n is an integer greater than 1.
6. The composition of claim 1 , further comprising an adjuvant.
7. The composition of claim 1 , wherein the composition is an immunogenic composition or a diagnostic composition.
8. The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.
9. The composition of claim 1 , further comprising one or more antigens selected from the group consisting of a protein antigen from Helicobacter pylori ; a protein antigen from N. meningitidis serogroup B; an outer-membrane vesicle (OMV) preparation from N. meningitidis ; a saccharide antigen from N. meningitidis serogroup A, C, W135 and/or Y; a saccharide antigen from Streptococcus pneumoniae ; an antigen from hepatitis A virus; an antigen from hepatitis B virus; an antigen from hepatitis C virus; an antigen from Bordetella pertussis ; a diphtheria antigen; a tetanus antigen; a saccharide antigen from Haemophilus influenzae B; an antigen from N. gonorrhoeae ; an antigen from Chlamydia pneumoniae ; an antigen from Streptococcus agalactiae ; an antigen from Streptococcus pyogenes ; an antigen from Chlamydia trachomatis ; an antigen from Porphyromonas gingivalis ; polio antigen(s); rabies antigen(s); measles, mumps and/or rubella antigens; influenza antigen(s); and an antigen from Moraxella catarrhalis.