Seneca valley virus based compositions and methods for treating disease
The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.
1. A method of killing an abnormally proliferative cell comprising contacting the cell with an isolated Seneca Valley virus or derivative or relative thereof, having a genome comprising a sequence that is at least 95% identical to SEQ ID NO:168, and wherein the abnormally proliferative cell is a neuroendocrine tumor cell.
2. The method of claim 1 , wherein the isolated Seneca Valley virus is the virus having ATCC Patent Deposit number PTA-5343.
3. The method of claim 1 , wherein the neuroendocrine tumor cell is a human neuroendocrine tumor cell.
4. The method of claim 3 , wherein the human neuroendocrine tumor cell is selected from the group consisting of: a small cell lung cancer (SCLC) cell, a large cell neuroendocrine carcinoma cell, a neuroblastoma, a Wilms' tumor cell, a retinoblastoma, a medulloblastoma, a non-SCLC tumor cell, a carcinoid tumor cell, and an adrenocortical carcinoma cell.
5. The method of claim 1 , wherein the isolated Seneca Valley virus is the virus having ATCC Patent Deposit number PTA-5343, and wherein the abnormally proliferative cell is a human neuroendocrine tumor cell selected from the group consisting of: a small cell lung cancer (SCLC) cell, a large cell neuroendocrine carcinoma cell, a neuroblastoma, a Wilms' tumor cell, a retinoblastoma, a medulloblastoma, a non-SCLC tumor cell, a carcinoid tumor cell, and an adrenocortical carcinoma cell.