Controlling zoonotic disease vectors from insects and arthropods using preconidial mycelium and extracts of preconidial mycelium from entomopathogenic fungi
View Patent ↗The present invention utilizes extracts of the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect and arthropod attractants and/or pathogens and can be employed to limit the zoonotic diseases they transmit. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material and extracts can be made thereof. More than one fungus and substrate can be used in combination with one or more antimicrobial, antiprotozoal, antiviral, and genetically modified agents that result in reduced spread of contagions and lessens the damage they inflict on animals, and plants.
1. A method comprising presenting an effective attracting amount of a preconidial preparation of an entomopathogenic fungus to attract and control arthropods that carry zoonotic diseases, wherein the preconidial preparation is selected from the group consisting of preconidial mycelium, extract of preconidial mycelium and both preconidial mycelium and extract of preconidial mycelium, wherein non-sporulating sectors are selectively cultured to produce the preconidial mycelium, wherein the preconidial mycelium contains less than 100 conidia per gram of myceliated substrate, wherein the preconidial preparation of an entomopathogenic fungus is blended with mushroom preparations selected from the group consisting of mycelium of Fomitopsis officinalis, Fomitopsis pinicola, Fomitporia robustus, Piptoporus betulinus, Trametes versicolor, Trametes elegans, Ganoderma lucidum, Ganoderma applanatum, Ganoderma annularis, Ganoderma oregonense, Ganoderma resinaceum, Ganoderma tsugae, Heterobasidion annosum, Inonotus obliquus, Antrodia camphorate, Rigidoporus ulmarius, Perenniporia fraxinophila, Psilocybe cyanescens, Psilocybe azurescens and Psilocybe cubensis mushrooms, extracts of the mycelium of said mushroom and combinations thereof, and wherein the arthropods are selected from the group consisting of insects, arachnids and myriapods.
2. The method of claim 1 wherein the insects are selected from the group consisting of malaria-carrying mosquitoes, virus-carrying mosquitoes, contagion-carrying flies, contagion-carrying ants, contagion-carrying bedbugs, contagion-carrying fleas and contagion-carrying midges and the arachnid is selected from the group consisting of virus-carrying ticks and contagion-carrying ticks.
3. The method of claim 1 wherein the entomopathogenic fungus is selected from the group consisting of the Deuteromycetes Metarhizium, Beauveria, Paecilomyces, Hirsutella, Verticillium, Culicinomyces, Nomuraea and Aspergillus , the Ascomycetes Cordyceps, Ophiocordyceps species and their anamorphs, Ascosphaera, Torrubiella, Hypocrella and its Aschersonia anamorph and combinations thereof.
4. The method of claim 1 wherein the entomopathogenic fungus is selected from the group consisting of Metarhizium anisopliae, Metarhizium flaviridq Beauveria bassiana, Beauveria brongniartii, Paecilomyces farinosus, Paecilomyces fumosoroseus, Verticillium lecanii Hirsutella citriformis, Hirsutella thompsoni, Aschersonia aleyrodis, Entomophaga grylli, Entomophaga maimaiga, Entomophaga muscae, Entomophaga praaxibulli, Entomophthora plutellae, Zoophthora radicans, Neozygites floridana, Nomuraea rileyi, Pandora neoaphidis, Tolypocladium cylindrosporum, Culicinomyces clavosporus, Lagenidium giganteum, Cordyceps variabilis, Cordyceps facis, Cordyceps (Ophiocordyceps) subsessilis, Cordyceps myrmecophila, Cordyceps sphecocephala, Cordyceps entomorrhiza, Cordyceps gracilis, Cordyceps militaris, Cordyceps washingtonensis, Cordyceps melolanthae, Cordyceps ravenelii, Cordyceps unilateralis, Cordyceps sinensis, Cordyceps clavulata , and combinations thereof.
5. The method of claim 1 wherein the entomopathogenic fungus is selected from the group consisting of Cordyceps sinensis, Cordyceps subsessilis, Cordyceps militaris, Cordyceps unilateralis, Ophiocordyceps species and their anamorphs including Metarhizium, Beauveria, Paecilomyces, Hirsutella, Beauveria and combinations thereof, wherein the insects are selected from the group consisting of mosquitoes, flies, bed bugs, fleas, midges and other insects and wherein the arthropods are selected from the group consisting of spiders, ticks and mites.
6. The method of claim 1 wherein the insects and the arthropods that carry zoonotic diseases are attracted to a locus for control.