IP Library Granted Patent US 8,753,698
Granted Patent B2
US 8,753,698 · App. 11/632,889 · Granted Jun 17, 2014

Anti-hypertensive functional food products

Inventors: Aart Van Amerongen (Veenendaal, NL); Maria Josepha Catharina Beelen-Thomissen (Oss, NL); Arie Van der Bent (Tiel, NL); Jan Hendrik Buikema (Groningen, NL); Wiekert Hendrikus Van Gilst (Haren, NL); Maria Henrieette Johanna Loonen (Oirlo, NL); Karin Beatrice Merck (Oss, NL); Jos Nelissen (Oirlo, NL); Wilhelmus Johannes Gertrudes Thielen (Venray, NL); Klaas Arnoud Togtema (Uden, NL)
Assignee: Globus Egg Sciences B.V.
A61K35/54A23L1/3053A23J3/341A61K38/38A61K38/47A23V2002/00A61K38/40A61K38/012
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Quick Facts
Patent No.
US 8,753,698
App. No.
11/632,889
Granted
Jun 17, 2014
Kind
B2
Abstract

The present invention provides novel protein hydrolysates with anti-hypertensive properties, as well as food products and food supplements comprising these.

Claims (16)

1. A food product or food supplement, comprising a Bacillius licheniformi endoproteinase hydrolysate of a lysozyme, wherein at least 30% of the hydrolysate are peptides of the lysozyme that have a molecular weight of less than 0.5 kD.

2. An enzymatic protein hydrolysate, wherein said hydrolysate is obtained by Bacillius licheniformi endoproteinase hydrolysis of a lysozyme and wherein at least 30% of the hydrolysate are peptides of the lysozyme that have a molecular weight of less than 0.5 kD.

3. A method for making a protein hydrolysate with anti-hypertensive activity, comprising the steps of:

(a) providing a liquid solution comprising a lysozyme;

(b) adjusting the pH of said solution to a pH suitable for Bacillius licheniformi endoproteinase; and

(c) adding Bacillius licheniformi endoproteinase to said solution and incubating the solution for a suitable period at a suitable temperature to obtain a hydrolysate of the lysozyme wherein at least 30% of the hydrolysate are peptides of the lysozyme that have a molecular weight of less than 0.5 kD.

4. The method according to claim 3 , further comprising a step (e) of filtering said solution through a membrane filter and obtaining a permeate which is enriched for active target peptides.

5. An anti-hypertensive protein hydrolysate, said hydrolysate obtained by the method according to claim 3 .

6. The food product or food supplement according to claim 1 , wherein said product or supplement is in the form of a liquid, solid or semi-solid.

7. A food product or food supplement, said food product or food supplement comprising the hydrolysate according to claim 5 .

8. The enzymatic protein hydrolysate according to claim 2 , wherein the proportion of di- and/or tri peptides in the hydrolysate is at least 50%.

9. The enzymatic protein hydrolysate according to claim 2 , wherein at least 50% of the hydrolysate are peptides that have a molecular weight of less than 0.5 kD.

10. The food product or food supplement according to claim 1 , wherein the proportion of di- and/or tri peptides of lysozyme in the hydrolysate is at least 50%.

11. The food product or food supplement according to claim 1 , wherein at least 50% of the hydrolysate are peptides that have a molecular weight of less than 0.5 kD.

12. A method of reducing blood pressure or reducing the risk of developing high blood pressure comprising administering a food product or food supplement comprising a Bacillius licheniformi endoproteinase hydrolysate of a lysozyme, wherein at least 30% of the hydrolysate are peptides of the lysozyme that have a molecular weight of less than 0.5 kD.

13. A food product or food supplement comprising the enzymatic protein hydrolysate according to claim 2 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 22, 2008
From: VAN AMERONGEN, AART; BEELEN-THOMISSEN, MARIA JOSEPHA CATHARINA; VAN DER BENT, ARIE; BUIKEMA, JAN HENDRIK; VAN GILST, WIEKERT HENDRIKUS; LOONEN, MARIA HENRIEETTE JOHANNA; MERCK, KARIN BEATRICE; NELISSEN, JOSEPH WILHELMUS PETRUS MARIA; THIELEN, WILHELMUS JOHANNES GERTRUDES; TOGTEMA, KLAAS ARNOUD
To: GLOBUS EGG SCIENCES B.V.
Reel/Frame 021430/0024 →
Priority Claims (1)
EP 05100544 · Jan 27, 2005 · regional
Continuity (2)
Provisional Application 60589902 · Jul 22, 2004
Related Publication 20090029005A1 · Jan 29, 2009