Polypeptides having xylanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide, which has at least 95% sequence identity to the sequence of amino acids 20 to 334 of SEQ ID NO: 2; or is a fragment of SEQ ID NO: 2, wherein the polypeptide has xylanase activity.
2. The polypeptide of claim 1 , having at least 96% sequence identity to the sequence of amino acids 20 to 334 of SEQ ID NO: 2.
3. The polypeptide of claim 1 , having at least 97% sequence identity to the sequence of amino acids 20 to 334 of SEQ ID NO: 2.
4. The polypeptide of claim 1 , having at least 98% sequence identity to the sequence of amino acids 20 to 334 of SEQ ID NO: 2.
5. The polypeptide of claim 1 , having at least 99% sequence identity to the sequence of amino acids 20 to 334 of SEQ ID NO: 2.
6. The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii).
7. The polypeptide of claim 1 , which is a variant of the mature polypeptide of SEQ ID NO: 2, comprising a substitution, deletion, and/or insertion at one or more positions.
8. The polypeptide of claim 1 , which comprises the sequence of amino acids 20 to 334 of SEQ ID NO: 2.
9. The polypeptide of claim 1 , which is a fragment of SEQ ID NO: 2.
10. A composition comprising the polypeptide of claim 1 and one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, a GH61 polypeptide, an expansin, an esterase, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
11. A process for degrading a cellulosic material, comprising: treating the cellulosic material with a polypeptide of claim 1 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide.
12. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a polypeptide of claim 1 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.
13. The process of claim 12 , further comprising recovering the fermentation product.
14. A process for degrading a cellulosic material, comprising: treating the cellulosic material with a polypeptide of claim 3 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide.
15. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a polypeptide of claim 3 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.
16. The process of claim 15 , further comprising recovering the fermentation product.
17. A process for degrading a cellulosic material, comprising: treating the cellulosic material with a polypeptide of claim 5 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide.
18. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a polypeptide of claim 5 , an endoglucanase, a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.
19. The process of claim 18 , further comprising recovering the fermentation product.