Antibody identifying an antigen-bound antibody and an antigen-unbound antibody, and method for preparing the same
View Patent ↗The present invention relates to an antibody that recognizes a first antibody, the antibody specifically recognizing one of a free first antibody and an antigen-binding first antibody. More specifically, the above antibody is a domino antibody that specifically recognizes and binds to an antigen-binding first antibody, or an antibody-unlocking antibody that specifically recognizes and binds to a free first antibody.
1. An isolated antibody, wherein the isolated antibody specifically recognizes a first antibody bound to fluorescein isothiocyanate (FITC).
2. A hybridoma deposited under ATCC accession number PTA-9167 or PTA-9168.
3. A method for obtaining a domino antibody, comprising:
combining a first antibody with a hapten or antigen,
chemically fixing or bonding the hapten or antigen to the antibody to prepare an antigen-antibody complex in which the hapten or antigen does not dissociate from the antibody, and
immunizing a host with the complex,
obtaining at least one hybridoma from the spleen cells of the host, wherein the hybridoma produces monoclonal antibodies, and
obtaining a domino antibody from the monoclonal antibodies produced by the obtained hybridoma,
wherein the domino antibody specifically recognizes conformational changes in the first antibody when the first antibody is bound to the hapten or antigen.
4. The method of claim 3 , wherein the antigen comprises at least one of gamma globulin, bovine serum albumin, fluorescein isothiocyanate (FITC), and (4-hydroxy-3-nitrophenyl)acetyl (NP).
5. The method of claim 3 , wherein the host is a mouse, a rat, a rabbit, a hamster, or a monkey.