Quantitave, multispectral image analysis of tissue specimens stained with quantum dots
A biological sample such as a tissue section is stained with one or more quantum dots and possibly other fluorophores (total number of fluorophores N). A camera coupled to a microscope generates an image of the specimen at a plurality of different wavelengths within the emission spectral band of the N fluorophores. An analysis module calculates coefficients C1 . . . CN at each pixel which are related to the concentration of each of the individual fluorophores. Morphological processing instructions find biological structures. A display module displays quantitative analysis results to the user. The quantitative analysis display includes histograms of the biological structures, scatter plots of fluorophore concentrations, statistical data, spectral data, image data and still others.
1. A workstation for quantitative analysis of a type of tissue specimen containing cells or cellular components stained with a plurality of fluorophores, at least one of which comprises a quantum dot, comprising:
a display for displaying quantitative analysis of the specimen and one or more images of the tissue specimen;
a processing unit; and
a memory storing software instructions for execution by the processing unit and multispectral image data acquired from the stained tissue specimen with the aid of a at least one of a microscope and a camera,
wherein the software instructions present on the display a user-selectable area for identifying, in the one or more images of the specimen, at least one of the plurality of fluorophores applied to the specimen, a user-selectable area for indicating whether the tissue specimen is to be analyzed using a reference spectrum for autofluorescence corresponding to the type of the tissue specimen, and a user selectable area for selecting the colors to represent, in the one or more images, the at least one the plurality of fluorophores present in the specimen for display purposes, and wherein the color selected for the at least one of the plurality of fluorophores is different from a color of the at least one of the plurality of fluorophores that was selected.
2. The apparatus of claim 1 , wherein the software instructions further provide a feature allowing a user to select via the display a segment of the one or more images of the specimen, and wherein the software instructions generate a display of quantitative results for the selected segment of the one or more images of the tissue specimen.
3. A network server generating quantitative analysis data for a remote workstation for quantitative analysis of a type of a tissue specimen containing cells or cellular components stained with a plurality of fluorophores, at least one of which comprises a quantum dot, the workstation having a display, the network server comprising:
a processing unit; and
a memory storing software instructions for execution by the processing unit and multispectral image data acquired from the stained specimen with the aid of at least one of a microscope and a camera,
wherein the software instructions present on the display a user-selectable area for identifying, in one or more images of the specimen, at least one of the plurality of fluorophores applied to the specimen, a user-selectable area for indicating whether the specimen is to be analyzed using a reference spectrum for autofluorescence corresponding to the type of the tissue specimen, and a user selectable area for selecting the colors to represent, in the one or more images, the at least one the plurality of fluorophores present in the specimen for display purposes, and wherein the color selected for the at least one of the plurality of fluorophores is different from a color of the at least one of the plurality of fluorophores that was selected.
4. A method for interfacing with a user interface for quantitative analysis of a type of tissue specimen stained with a plurality of fluorophores, at least one of which comprises a quantum dot, and wherein the tissue specimen is imaged after being stained, comprising:
visually displaying, on a display, a user-selectable area that allows a user to identify at least one of the plurality of fluorophores applied to the tissue specimen;
visually displaying, on the display, a user-selectable area that allows a user to select whether an image of the tissue specimen is analyzed using a reference spectrum for autofluorescence corresponding to the tissue type of the tissue specimen; and
visually displaying, on the display, a user selectable area for selecting the colors to represent, in the image, the plurality of fluorophores present in the specimen, and wherein a different color is selected for each of the plurality of fluorophores.
5. A workstation for quantitative analysis of a type of tissue specimen containing cells or cellular components stained with a plurality of fluorophores, comprising:
a display for displaying quantitative analysis of the specimen and one or more images of the tissue specimen;
a processing unit; and
a memory storing software instructions for execution by the processing unit and multispectral image data acquired from the stained tissue specimen with the aid of a at least one of a microscope and a camera,
wherein the software instructions present on the display a user-selectable area for identifying, in the one or more images of the specimen, at least one of the plurality of fluorophores applied to the specimen, a user-selectable area for indicating whether the tissue specimen is to be analyzed using a reference spectrum for autofluorescence corresponding to the type of the tissue specimen, and a user selectable area for selecting the colors to represent, in the one or more images, the at least one the plurality of fluorophores present in the specimen for display purposes, and wherein the color selected for the at least one of the plurality of fluorophores is different from a color of the at least one of the plurality of fluorophores that was selected.