Slicing device
The invention relates to a biological microglia comprising at least two pores having a size adopted to allow cells, cell aggregates, tissue or other biological material to pass through said pores, and one or several slicing beams separating said pores from each other, wherein biological material is split/sliced/cleaved into at least two parts when passing said microgrid, a slicing device, an apparatus comprising said slicing device as well as the use of said microgrid, slicing device and apparatus.
1. A tissue or cell aggregate slicing apparatus comprising a slicing device, the slicing device comprising at least one microgrid, the microgrid made of silica, ceramics, or metal and comprising 2 to 1000 pores having a pore size of 50 to 200 μm and 2 to 200 slicing beams separating said pores from each other, said slicing beams having a width of 2.5 to 40 micrometers, a depth that is equal to a thickness of the microgrid, and sharp upper and/or lower edges forming a cutting edge for slicing tissue or cell aggregate;
wherein tissue or cell aggregate larger than the pores is sliced into at least two parts when passing said microgrid to form cell clusters comprising viable cells, the formed cell clusters having a width and height corresponding to dimensions of the pores;
wherein said slicing device is embedded in a flow-through unit, said flow-through unit comprising a first and a second opening allowing a stream of tissue or cell aggregate to enter through said first opening, pass through the microgrid within the slicing device, and the resulting cell clusters exit through the second opening;
wherein the pores have a rectangular shape; and
wherein the cutting edge of the slicing beams is positioned in the stream to slice the tissue or cell aggregate into cell clusters as the stream passes through the microgrid.
2. A method of obtaining cell clusters from tissue or cell aggregates, comprising slicing the tissue or cell aggregates with a slicing apparatus according to claim 1 to obtain slices of cell clusters from the tissue or cell aggregates.
3. The slicing apparatus according to claim 1 , wherein the microgrid is comprised of silica.
4. The slicing apparatus according to claim 3 , wherein the microgrid comprises microstructures produced by wet or dry etching of a silicon wafer exposed and developed by photolithography.
5. A method for manufacturing a slicing apparatus according to claim 1 , comprising defining a desired projected shape of the microgrid by photolithography on a silicon wafer, and subsequently subjecting the silicon wafer to wet or dry etching to produce microstructures that compose the microgrid, such that the microgrid comprises about 2 to about 1000 pores having a pore size of 50 to 200 μm and 2-200 slicing beams separating said pores from each other, said slicing beams having a width ⅕ to 1/20 of the size of the pores and sharp upper and/or lower edges, and the pores having a rectangular shape.
6. The slicing apparatus according to claim 1 , comprising a pressure sensor to control fluid pressure through the microgrid to prevent deleteriously high pressure when aspirating the tissue or cell aggregate through the microgrid.