Assay plates, reader systems and methods for luminescence test measurements
Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
1. A multi-well plate comprising:
(a) a plate top comprising plate top openings; and
(b) a plate bottom mated to the plate top to define an array of wells of the multi-well plate corresponding to a 96-well plate configuration, wherein the plate bottom comprises a substrate having a top surface with a plurality of electrodes patterned thereon and a bottom surface with a plurality of electrical contact surfaces patterned thereon, wherein the plurality of electrodes and contact surfaces are electrically connected and patterned to define two or more independently addressable sectors within the array, wherein each sector comprises a set of two or more wells, and each of the sectors comprises fewer contact surfaces than wells in the sector.
2. The multi-well plate of claim 1 , wherein the plurality of electrodes comprise one or more working electrodes and one or more counter electrodes and the plurality of contact surfaces comprise one or more working contacts electrically connected to the one or more working electrodes and one or more counter contacts electrically connected to the one or more counter electrodes.
3. The multi-well plate of claim 1 , wherein the array comprises one or more of:
(i) a firs sector having wells A1 through A4, B1 through B4, C1 through C4, and D1 through D4;
(ii) a second sector having wells A5 through A8, B5 through B8, C5 through C8, and D5 through D8;
(iii) a third sector of wells A9 through A12, B9 through B12, C9 through C12 and D9 through D12;
(iv) a fourth sector of wells E1 through E4, F1 through F4, G1 thorough G4 and H1 through H4;
(v) a fifth sector of wells E5 through E8, F5 through F8, G5 through G8 and H5 through H8; and
(vi) a sixth sector of wells E9 through E12, F9 through F12, G9 through G12 and H9 through H12.
4. The multi-well plate of claim 3 , wherein the contact surfaces are located at one or more of:
(i) two or more of first sector locations: A1-B2; A2-B3; A3-B4; C1-D2; C2-D3; C3-D4;
(ii) two or more of second sector locations: A5-B6; A6-B7; A7-B8; C5-D6; C6-D7; C7D8;
(iii) two or more of third sector locations: A9-B10; A10-B11; A11-B12; C9-D10; C10-D11; C11-D12;
(iv) two or more of fourth sector locations: E1-F2; E2-F3; E3-F4; G1-H2; G2-H3; G3-H4;
(v) two or more of fifth sector locations: E5-F6; E6-F7; E7-F8; G5-H6; G6-H7; G7-H8; and
(vi) two or more of sixth sector locations: E9-F10; E10-F11; E11-F12; G9-H10; G10-H11; G11-H12.
5. The multi-well plate of claim 1 , wherein the electrodes comprise carbon particles.
6. The multi-well plate of claim 1 , wherein the electrodes comprise a printed conductive material.
7. The multi-well plate of claim 1 , wherein one or more of the electrodes comprises a plurality of assay domains formed thereon.
8. The multi-well plate of claim 7 , wherein the plurality of assay domains comprises at least four assay domains.
9. The multi-well plate of claim 7 , wherein the plurality of assay domains comprises at least seven assay domains.
10. The multi-well plate of claim 7 , wherein the plurality of assay domains comprises at least ten assay domains.
11. The multi-well plate of claim 1 , further comprising a luminescent label in one or more of the wells.
12. The multi-well plate of claim 1 , wherein said plurality of contact surfaces are located between or adjacent said wells.
13. A kit for use in the conduct of an electrode-induced luminescence assay comprising, in one or more containers, a multi-well assay plate of claim 1 and at least one electrochemiluminescent label.
14. The kit of claim 13 , wherein said electrochemiluminescent label is a ruthenium or osmium-containing organometallic compound.
15. The kit of claim 14 , wherein said electrochemiluminescent label comprises tris-bipyridyl-ruthenium.
16. The kit of claim 13 , wherein said electrochemiluminescent label is attached to a protein.
17. The kit of claim 13 , wherein said electrochemiluminescent label is attached to an antibody.
18. The kit of claim 13 , wherein said electrochemiluminescent label is attached to a nucleic acid.
19. The kit of claim 13 , further comprising one or more assay components, wherein at least one of said assay components is immobilized within said wells.
20. The kit of claim 19 , wherein said immobilized assay component is dried within said well.
21. The kit of claim 13 wherein said kit further comprises a binding reagent which binds a component of an assay conducted in said multi-well plate.
22. The kit of claim 21 , wherein said binding reagent is a protein.
23. The kit plate of claim 21 , wherein said binding reagent is an antibody.
24. The kit of claim 21 , wherein said binding reagent is a nucleic acid.
25. The kit of claim 24 wherein said nucleic acid binds to a complementary nucleic acid linked to an assay component.