Helper virus-free AAV production
A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
1. A method for efficiently producing substantially helper-virus free stocks of recombinant adeno-associated virus comprising:
(a) transfecting cells permissive for adenovirus-associated virus replication with a helper virus vector which provides the helper-viral functions essential for a productive adeno-associated virus infection but which cannot itself be packaged into infectious helper virus virions;
(b) infecting said cells with recombinant adeno-associated virus; and
(c) collecting virions produced, wherein the collected virions form a substantially helper-virus free stock of recombinant adeno-associated virus, wherein the production of recombinant adeno-associated virus is comparable to that provided by a method using helper virus infection.
2. A method for efficiently producing substantially helper-virus free stocks of recombinant adeno-associated virus comprising:
(a) transfecting cells permissive for adenovirus-associated virus replication with a helper virus vector which provides the helper-viral functions essential for a productive adeno-associated virus infection but which cannot itself be packaged into infectious helper virus virions; and
(b) transfecting said cells with infectious recombinant adeno-associated virus DNA; and
(c) collecting virions produced, wherein the collected virions form a substantially helper-virus free stock of recombinant adeno-associated virus, wherein the production of recombinant adeno-associated virus is comparable to that provided by a method using helper virus infection.
3. A method for efficiently producing substantially helper-virus free stocks of recombinant adeno-associated virus comprising:
(a) cotransfecting cells permissive for adenovirus-associated virus replication, wherein said cells comprise an extrachromosomal element comprising: a helper virus vector which provides the helper viral functions essential for a productive adeno-associated virus infection; and an inducible promoter, with:
i) a recombinant adeno-associated virus vector which is capable of being packaged into infectious AAV virions, and
ii) a helper AAV vector which provides the AAV-viral functions essential for the replication and packaging of said recombinant adeno-associated virus vector into infectious AAV virions; and
(b) collecting virions produced, wherein the collected virions form a substantially helper-virus free stock of recombinant adeno-associated virus, wherein the production of recombinant adeno-associated virus is comparable to that provided by a method using helper virus infection.
4. The method of claim 1 , wherein the cells are infected with recombinant human adeno-associated virus, comprising producing substantially helper-virus free recombinant human adeno-associated virus stocks.
5. The method of claim 2 , wherein the cells are transfected with recombinant human adeno-associated virus, comprising producing substantially helper-virus free recombinant human adeno-associated virus stocks.
6. The method of claim 3 , wherein the cells are co-transfected with recombinant human adeno-associated virus, comprising producing substantially helper-virus free recombinant human adeno-associated virus stocks.