IP Library Granted Patent US 8,846,306
Granted Patent B2
US 8,846,306 · App. 12/672,610 · Granted Sep 30, 2014

Use of a globin, a globin protomer or an extracellular hemoglobin obtained from the marine worm,

Inventors: Franck Zal (Ploujean-Morlaix, FR); Morgane Rousselot (Saint Pol de Leon, FR); Delphine Dutheil (Saint Julien l'Ars, FR)
Assignees: Hemarina SA; Centre National de la Recherche Scientifique; Universite Pierre et Marie Curie (Paris 6)
A01N1/0226C12N5/0037A61K38/42
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Quick Facts
Patent No.
US 8,846,306
App. No.
12/672,610
Granted
Sep 30, 2014
Kind
B2
Abstract

The invention relates to the use of at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin for the preservation of organs, tissues, organ and tissue cells and cell cultures.

Claims (25)

1. A method for the preservation of organs, tissues, or organ cells comprising perfusing said organs, tissues, or organ cells with a composition comprising at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin of an invertebrate animal selected from a marine worm of the phylum Annelida, in combination with an organ storage medium, said medium being capable of protecting said organs, tissues, or cells from the deleterious effects of ischemia while satisfying the minimum metabolic needs of said organs, tissues, or cells wherein said marine worm is Arenicola marina.

2. The method of claim 1 , characterized in that the organ storage medium is selected from the group consisting of University of Wisconsin solution (UW, Viaspan®), IGL 1®, Celsior®, SCOT Maco®, BMPS Belzer®, Custodiol® (HTK), Euro-Collins®, Soltran®, Perfadex®, Ringer Lactate®, and Plegisol®.

3. The method of claim 1 , wherein said tissues or organ cells comprise vertebrate cells.

4. The method of claim 1 , wherein said tissues or organ cells comprise invertebrate cells.

5. The method of claim 4 , wherein the temperature of the composition is from about 4° C. to about 15° C.

6. The method of claim 1 , wherein the temperature of the composition is from about 4° C. to about 15° C.

7. The method of claim 1 , wherein the temperature of the composition is from about 25° C. to about 37° C.

8. The method of claim 1 , wherein the at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin is at a concentration of about 0.625 mg/ml to about 100 mg/ml, relative to the final volume of the composition.

9. A process for storage of organs, tissues, or organ cells, the process comprising static or dynamic perfusion storage of said organs, tissues, or cells comprising:

perfusing said organs, tissues, or cells with a composition comprising at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin of an invertebrate animal selected from a marine worm of the phylum Annelida, in combination with an organ storage medium, said medium being capable of protecting said organs, tissues, or cells from the deleterious effects of ischemia while satisfying the minimum metabolic needs of said organs, tissues, or cells wherein said marine worm is Arenicola marina.

10. The process of claim 9 , further comprising:

rinsing of said organ, tissue, or organ cells, previously removed from an organism, with said composition at a temperature of about 4° C. to about 15° C.; and

performing the static or dynamic perfusion storage at a temperature of about 4° C. to about 15° C., for a defined time.

11. The process of claim 9 further comprising:

rinsing of said organ, previously removed from an organism, at a temperature of about 25° C. to about 37° C. with said composition; and

performing the static or dynamic perfusion storage of said organ with said composition at a temperature of about 25° C. to about 37° C. for a defined time.

12. The method of claim 9 , wherein the at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin is at a concentration of about 0.625 mg/ml to about 100 mg/ml, relative to the final volume of the composition.

13. A process for culturing organ cells or organ tissues of vertebrates or of invertebrates, the process comprising culturing said tissues or cells in the presence of a composition comprising at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin of an invertebrate animal selected from a marine worm of the phylum Annelida, in combination with an organ storage medium or a cell culture medium, said medium being capable of protecting said tissues or cells from the deleterious effects of ischemia while satisfying the minimum metabolic needs of said tissues or cells wherein said marine worm is Arenicola marina.

14. The process of claim 13 , comprising:

culturing organ cells at a temperature from about 25° C. to about 37° C., for a defined time; and

harvesting the cells by rinsing and lysing the cells.

15. The process of claim 13 , comprising:

culturing organ cells at a temperature from about 4° C. to about 15° C., for a defined time; and

harvesting the cells by rinsing and lysing the cells.

16. The method of claim 13 , wherein the at least one globin and/or at least one globin protomer and/or at least one naturally extracellular native hemoglobin is at a concentration of about 0.625 mg/ml to about 100 mg/ml, relative to the final volume of the composition.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 28, 2010
From: ZAL, FRANCK; ROUSSELOT, MORGANE; DUTHEIL, DELPHINE
To: HEMARINA SA; CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE; UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)
Reel/Frame 024303/0035 →
Continuity (1)
Related Publication 20100209902A1 · Aug 19, 2010