Non-ionically bound constructs, and compositions and kits comprising domains
The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits.
1. A nucleic acid construct comprising at least three nucleic acid strands:
(a) a first nucleic acid strand, wherein said first nucleic acid strand is a circular strand;
(b) a second nucleic acid strand, wherein said second nucleic acid strand is fully complementary to said first nucleic acid strand;
(c) a third nucleic acid strand comprising a first portion complementary to said first nucleic acid strand and a second portion not complementary to said first nucleic acid strand and said second nucleic acid strand,
wherein when said first nucleic acid strand is hybridized to said second nucleic acid strand to form a double stranded portion, said double-stranded portion forms a template for synthesis of a nucleic acid product when present in a cell;
wherein when said first nucleic acid strand is hybridized to said first portion of said third nucleic acid strand, said second nucleic acid strand and said first portion of said third nucleic acid strand form a gapped circle, and said second portion of said third nucleic acid strand forms a linear tail, wherein
said linear tail is covalently attached to an antibody or is hybridized a fourth nucleic acid strand that is covalently attached to an antibody.
2. The construct of claim 1 wherein said antibody comprises a polyclonal or monoclonal antibody.