Multilayer fibrous polymer scaffolds, methods of production and methods of use
Fibrous polymer scaffolds and methods of manufacture are provided. The scaffolds can be formed of multiple layers and the extent and direction of alignment of each layer can be controlled. Efficient fabrication systems and methods for producing such scaffolds include apparatuses and processes are also provided. Kits including the fibrous polymer scaffolds and methods for implanting such scaffolds are also provided.
1. A fibrous polymer scaffold, comprising:
a first layer of aligned polymer fibers, the fibers comprising poly(L-lactide-co-caprolactone) and poly(propylene glycol); and
a second layer of unaligned polymer fibers, the fibers comprising poly(L-lactide-co-caprolactone) and poly(propylene glycol);
wherein poly(propylene glycol) is present in an amount of 0.1%-10% of the total weight of the polymer fibers.
2. The fibrous polymer scaffold of claim 1 ,
wherein at least one polymer fiber in the first layer and at least one polymer fiber in the second layer is the same polymer fiber that is continuous between the layers.
3. The fibrous polymer scaffold of claim 1 , wherein the scaffold is a shape selected from a membrane and a conduit.
4. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the first layer are aligned parallel to a first axis of the scaffold, the first axis being parallel to a longitudinal axis of a collector used to create the scaffold.
5. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the first layer are longitudinally aligned.
6. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the second layer are randomly oriented.
7. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the second layer are aligned parallel to a second axis of the scaffold.
8. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the second layer are circumferentially aligned.
9. The fibrous polymer scaffold of claim 1 , wherein the polymer fibers of the first layer and the polymer fibers of the second layer comprise a material selected from an aliphatic polyester, a poly(alkylene) oxide, poly(L-lactic acid), 70/30 L-lactide/e-caprolactone co-polymer, poly(caprolactone), poly(DL-lactide-co-caprolactone), poly(D-lactide-co-caprolactone), poly(L-lactide-co-caprolactone), poly(lactide-co-glycolic acid), poly(vinylpyrrolidine), poly(dimethylsiloxane), poly(lysine), collagen, laminin, fibronectin, elastin, alginate, fibrin, hyaluronic acid, proteoglycans, polypeptides, poly(ethylene-co-vinyl) alcohol, poly(vinyl) alcohol, poly(ethylene) glycol, poly(propylene) glycol, poly-L-lactide-co-glycolide-co-c-ε-caprolactone, poly(tetrafluoroethylene), poly(dioxanone), polyglactin 910 and combinations thereof;
wherein the aliphatic polyester is selected from D-lactide, L- lactide, poly(lactic acid), poly(lactide) glycolic acid, poly(glycolic acid), poly(glycolide), glycolide, poly(lactide-co-glycolide), poly(lactic acid-co-glycolic acid), epsilon-caprolactone, poly(epsilon-caprolactone), poly(lactide-co-caprolactone) and poly(glycolide-co-caprolactone) and combinations thereof; and
wherein the poly(alkylene) oxide is selected from poly(ethylene) oxide and poly(propylene) oxide.
10. The fibrous polymer scaffold of claim 1 , comprising an additive selected from poly(ethylene oxide), triethyl citrate, glycerol, poly(ethylene glycol), and combinations thereof.
11. The fibrous polymer scaffold of claim 1 , comprising a salt selected from NaCI, KH 2 PO 4 , K 2 HPO 4 , KIO 3 , KCI, MgSO 4 , MgCl 2 , NaHCO 3 , CaCl 2 sodium acetate, salt of acetic acid, salt of ascorbic acid, salt of citric acid, salt of lactic acid, salt of glycolic acid, and combinations thereof.
12. The fibrous polymer scaffold of claim 1 , wherein the at least one continuous polymer fiber comprises sodium acetate.
13. A method of implanting a fibrous polymer scaffold, comprising: contacting a defect in a tissue of a subject with a first layer of a fibrous polymer scaffold according to claim 1 , so that the scaffold at least partially replaces the defect; and
securing the scaffold in place at the defect.
14. A kit for the repair of wounds, surgical incisions or biopsies comprising:
(i) a fibrous polymer scaffold according to claim 1 ; and
(ii) instructions for using the scaffold to repair wounds, surgical incisions or biopsies by promoting the regeneration of at least one anatomical biological component selected from abdominal wall, amniotic sac, bladder, blood vessels, brain, cardiac tissue, fascia, gingival tissue, intestine, ligaments, meningeal tissue, mucous membranes, muscle, nerves, skin, spinal cord, stomach, and tendons.