Quantum dot barcode structures and uses thereof
View Patent ↗The present invention provides novel barcode microstructures and methods for making and using the microstructures for molecular recognition, and/or delivery of therapeutic, diagnostic, contrast, and imaging agents.
1. A microstructure comprising
(a) a core; and
(b) a plurality of distinct layers surrounding the core; wherein the plurality of distinct layers comprises (i) quantum dot-containing layers, and (ii) spacer layers not containing quantum dots, wherein there are at least two quantum dot containing layers; wherein the quantum dots are present in each quantum dot containing layer at a defined intensity level, wherein each quantum dot containing layer comprises a plurality of a single type of quantum dot that is different relative to quantum dots contained in any of the other quantum dot containing layers, wherein each quantum dot-containing layer is separated from other quantum dot containing layers by a spacer layer, and wherein a ratio of fluorescent intensities from the quantum dots in the plurality of quantum dot containing layers after simultaneous excitation defines a unique barcode for the microstructure.
2. The microstructure of claim 1 , wherein the microstructure is between 100 nm and 5 mm in diameter.
3. The microstructure of claim 1 wherein the microstructure is between 100 nm and 2 mm in diameter.
4. The microstructure of claim 1 wherein the microstructure comprises at least four_quantum dot-containing layers.
5. The microstructure of claim 1 wherein the microstructure comprises at least three quantum dot-containing layers.
6. The microstructure of claim 4 wherein the spacer layers are between 10 nm and 500 nm in thickness.
7. The microstructure of claim 1 , wherein the quantum dots are covalently embedded in the quantum-dot containing layers.
8. The microstructure of claim 1 , wherein the core comprises superparamagnetic material.
9. The microstructure of claim 8 , wherein the superparamagnetic material is selected from the group consisting of Fe 3 O 4 nanoparticles, Fe 2 O 3 nanoparticles, Mg/Co/Mn doped iron oxide nanoparticles, and FePt nanoparticles.
10. The microstructure of claim 8 , wherein the microstructure comprises a spacer layer coated over the core.
11. The method of claim 1 , wherein the plurality of distinct layers comprise silica-containing layers.
12. The microstructure of claim 1 wherein the quantum dots are selected from the group consisting of CdS quantum dots, CdSe quantum dots, CdSe@CdS core/shell quantum dots, CdSe@ZnS core/shell quantum dots, CdTe quantum dots, PbS quantum dots, and PbSe quantum dots.
13. The microstructure of claim 1 further comprising one or more probes attached to an outer surface of the microstructure.
14. The microstructure of claim 13 wherein the probe comprises a molecule selected from the group consisting of nucleic acids, proteins, antibodies, polysaccharides, lipids, organic molecules, and inorganic molecules.
15. A molecular recognition system comprising a plurality of different populations of the microstructures of claim 13 , wherein each population has a unique bar-code based on the quantum dots present in the microstructure.
16. A method for molecular recognition comprising:
(a) contacting the molecular recognition system of claim 15 with a test sample under conditions suitable for binding of the one or more probes to a target if present in the test sample;
(b) removing unbound microstructures;
(c) detecting fluorescence from microstructures bound to the test sample; and
(d) identifying presence of one or more targets in the test sample based on a ratio of fluorescence intensity emissions from microstructures bound to the test sample.
17. A method for making the microstructure of claim 1 , comprising:
(a) providing a core;
(b) coating the core with a plurality of distinct layers, wherein each layer is applied sequentially, wherein the plurality of distinct layers comprises (i) quantum dot-containing layers, and (ii) spacer layers not containing quantum dots, wherein there are at least two quantum dot containing layers; wherein the quantum dots are present in each quantum dot containing layer at a defined intensity level, wherein each quantum dot containing layer comprises a single type of quantum dot that is different relative to quantum dots contained in any of the other quantum dot containing layer, wherein each quantum dot-containing layer is separated from other quantum dot containing layers by a spacer layer, and wherein a ratio of fluorescent intensities from the quantum dots in the plurality of quantum dot containing layers after simultaneous excitation defines a unique barcode for the microstructure.
18. A method for delivering a therapeutic agent to a subject in need thereof, comprising administering the microstructure of claim 1 , wherein the core is hollow, and wherein the microstructure comprises a therapeutic agent loaded into the hollow core.
19. A method for delivering a diagnostic agent to a subject in need thereof, comprising administering the microstructure of claim 1 , wherein the core is hollow, and wherein the microstructure comprises a diagnostic agent loaded into the hollow core.
20. A method for delivering a contrast agent to a subject in need thereof, comprising administering the microstructure of claim 1 , wherein the core is hollow, and wherein the microstructure comprises a contrast agent loaded into the hollow core.
21. A method for delivering an imaging agent to a subject in need thereof, comprising administering the microstructure of claim 1 , wherein the core is hollow, and wherein the microstructure comprises an imaging agent loaded into the hollow core.